Studies on Participation of Bacteria on Hands of Hospital Staff to Nosocomial Infections

医院工作人员手部细菌参与医院感染的研究

• 批准号: 11672318
• 负责人: KITAME Fumio
• 金额: $ 2.24万
• 依托单位: Yamagata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11672318/
• 关键词: Nosocomial Infection; Bacteria; Contamination of Hand; MRSA; メチシリン耐性黄色ブドウ球菌; DNA型別解析; コアグラーゼ型; PFGE

To elucidate the participation of methicillin-resistant Staphylococcus aureus (MRSA) on hands of medical staffs in the dissemination of the MRSA among patients, a total of 215 isolates from patients and medical staffs in 5 different wards of Yamagata university hospital were investigated by DNA typing, coagulase typing, antibiogram and clinical informations.1. Nosocomial infections by 4-6 different DNA types were prevalent in every 5 wards between 1995 and 1997.2. One hundred and thirty three persons of patients and medical staffs were involved in the nosocomial infections.3. The some strains with specific DNA type were disseminated to several different wards.4. It was sugested that medical treatment by staffs in intensive care unit and moving of patients from ward to ward participated strongly in the disseminations of the MRSA.5. There were 2 types of MRSA isolates, that is, endemic strains which were disseminated among many patients, and none endemic strains which were isolated only from 1-2 patients.6. All of 215 strains of MRSA were sensitive to 4 antiseptics commonly used in this university hospital.7. Although the probability that MRSA strains isolated from the same patient at various intervals were the same clone was so high, it was also shown that in some cases, different clone based on mutation and reinfection has been isolated from the same patients.8. Publications : 1)Kankyou-kansen ; 15, 285-290, 2000 (in japanese)2) Kankyou-kansen ; 15, 295-305, 2000 (in japanese)

为了阐明医务人员手上的耐甲氧西林金黄色葡萄球菌（MRSA）参与MRSA在患者中传播的过程，对山形大学附属医院5个不同病区的患者和医务人员共215株分离株进行了DNA分型调查、凝固酶分型、抗菌谱和临床信息。 1． 1995年至1997.2年间，每5个病房就有4-6种不同DNA类型的院内感染流行。院内感染患者及医务人员133人。 3．一些具有特定DNA类型的菌株被传播到几个不同的病区。4．据推测，重症监护室工作人员的医疗以及患者从一个病房转移到另一个病房对 MRSA 的传播有很大影响。5。 MRSA分离株有2种类型，即在许多患者中传播的地方性菌株和仅在1-2名患者中分离到的非地方性菌株。 6． 215株MRSA均对该校医院常用的4种抗菌药物敏感。7．尽管在不同时间间隔从同一患者中分离到的MRSA菌株为同一克隆的可能性很高，但也表明在某些情况下，从同一患者中分离到基于突变和再感染的不同克隆。8．出版物 : 1) 交通干线 ; 15, 285-290, 2000（日语）2) Kankyou-kansen ; 15, 295-305, 2000（日语）

项目成果

Kitame F., Ohori N.: "Genetical and Bacteriological Nature of Methicillin-resistant Staphylococcus aureus Isolated from Same Patients at Various Intervals.(in Japanese)"Kankyou kansen. 15(4). 285-290 (2000)

Kitame F.、Ohori N.：“不同时间间隔从同一患者中分离出的耐甲氧西林金黄色葡萄球菌的遗传和细菌学性质。（日语）”Kankyou kansen。

Ohori N., Kitame F.: "Bacteriological and Epidemiological Analysis of Nosocomial Infection Caused by Methicillin-resistant Staphylococcus aureus in a University Hospital.(in Japanese)"Kankyou kansen. 15(4). 295-305 (2000)

Ohori N.，Kitame F.：“大学医院耐甲氧西林金黄色葡萄球菌引起的医院感染的细菌学和流行病学分析。（日语）”Kankyou kansen。

大堀直美,北目文郎: "大学病院におけるメチシリン耐性黄色ブドウ球菌による院内感染の細菌学的並びに疫学的解析."環境感染. 15・4. 295-305 (2000)

Naomi Ohori，Fumio Kitame：“大学医院耐甲氧西林金黄色葡萄球菌引起的医院感染的细菌学和流行病学分析”15・4（2000）。

北目文郎,大堀直美: "同一患者から間隔を置いて分離されたメチシリン耐性黄色ブドウ球菌の遺伝学的および細菌学的性状."環境感染. 15・4. 285-290 (2000)

Fumio Kitame、Naomi Ohori：“从同一患者中间隔分离出的耐甲氧西林金黄色葡萄球菌的遗传和细菌学特征。” 15・4 (2000)。

北目文郎,大堀直美: "同一患者から間隔を置いて分離されたメチシリン耐性黄色ブドウ球菌の遺伝学的および細菌学的性状"環境汚染. 15・4. 285-290 (2000)

Fumio Kitame、Naomi Ohori：“从同一患者中间隔分离出的耐甲氧西林金黄色葡萄球菌的遗传和细菌学特征”环境污染 15・4（2000）。