Examination of the histological and bacteriological features of the interface between bacterial deposits and root dentin using a device that included exposed root dentin reproduced pulpal pressure in vitro.

使用包含暴露的牙根牙本质的装置检查细菌沉积物和牙根牙本质之间的界面的组织学和细菌学特征，并在体外再现牙髓压力。

基本信息

批准号：
11671896
负责人：
OZAKI Kazumi
金额：
$ 2.24万
依托单位：
The University of Tokushima, School of Dentistry
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671896/
关键词：
Caries-associated bacteria Pulpal pressure Intracellular polysaccharide 菌体関連多糖

项目摘要

We evaluated structural change of the dentin and alteration of the localization and carbohydrate metabolism of bacteria in dental plaque and into dentinal tubules on the root dentin specimens in vitro and in situ, that we could observe with the progress in the time. In vitro experiments demonstrated that pH of the medium used for mixed culture with Streptococcus mutans and Lactobacillus casei was lower than it of the independent culture on a device which reproduced pulpal pressure by supplying the saline pressurized through dentinal tubules exposed in the pulpal side of root dentin specimens.On microscope analysis, the numbers of bacterially infected dentinal tubules in specimens used for the mixed culture was significantly more abounding than it of the independent culture. By periodate-thiocarbohydrazide-silver proteinate-physical development (PTSP) staining method, which could detect the intracellular polysaccharide in bacterial cells, it was clarified that much intracellular polysaccharide exists in most bacteria within which exist in microcolonies of plaque layer nearest the dentin surface and in which invaded into dentinal-tubules.On the other hand, in a root-dentin specimen, compulsorily exposed the surface and to the oral environment for only two months, the penetration of the bacteria into the dentinal tubules was visible, while in a twelve-month root-surface specimens in situ, some microcavities coalesced an indicated more wide-ranging cavities, but subsequent destruction of the dentin was not observed. The existence of mantle dentine was indicated as the factor by which brought about the difference of these results. It became clear to dynamically change in the time-dependent destruction of the dentin, which progresses on localization and abundance ratio of PTSP-positive-bacteria by means of image processing technology by the computer.

我们评估了牙本质的结构变化，并改变了牙菌斑中细菌的定位和碳水化合物代谢，并在根牙本质标本上的牙本质小管中，在体外和原位，我们可以观察到时间的进展。体外实验表明，用链球菌和乳杆菌甲壳虫混合培养的培养基的pH低于独立培养物的pH，在该装置上，通过在牙本质牙本质牙髓中暴露于牙本质的牙本质小管中供应盐水，从而重现果皮压力，该装置通过牙皮压力供应了果皮压力。在显微镜分析中，用于混合培养的标本中细菌感染的牙本质小管的数量比独立培养物的比例要大得多。通过周期硫代硫代乙酰氢氮酰基 - 丝质蛋白蛋白 - 物理发育（PTSP）染色方法，可以检测细菌细胞中细胞内多糖的细胞内多糖，因此，在大多数细菌中，大多数细菌都存在于大多数细菌中，其中大多数细菌都存在于denentin表面和牙本质表面的微小离子中，并且在另一方面，在根丁丁标本中，在其中侵入牙本质小管中，仅在两个月内强制暴露于表面和口腔环境中，将细菌渗透到牙本质小管中，而在十二个 - 月度表面标本的原地，一些微腔融合了一个指示的更广泛的腔，但未观察到随后破坏牙本质。地幔牙本质的存在被指示为导致这些结果差异的因素。很明显，可以动态地改变牙本的时间依赖性破坏，该破坏通过计算机通过图像处理技术来取决于PTSP阳性 - 细菌的定位和丰度比。