Induction of fertilization by intrapronuclear male gameteinjections into oocyte pronucleus

原核内雄配子注射至卵母细胞原核诱导受精

• 批准号: 11671554
• 负责人: NIKOLAOS Sofikitis
• 金额: $ 0.9万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671554/
• 关键词: oocvte; sperm; pronuclei; fertilization; 雌性前核; 雄性前核; ラット

Oocyte activation, sperm head decondensation within the ooplasm, transformation of the male gamete nucleus into the pronucleus and pronuclei fusion are the last steps of the fertilization process. failure in one of the above interactions between the male and the female gamete results in ICSI failure. We investigated the possibility of bypassing the last events of the fertilization process by injecting a pre-decondensed or preactivated oocytes.We evaluated the development of rat preactivated oocytes after intrapronuclear pre-decodensed sperm head injections (IPSHIs). maturn rat oocytes received a combination of electrical and chemical stimulation or a combination mechanical and chemical stimulation. Activated oocytes were treated with IPHSI.A pre-decondensed sperm head was injected into the larger female pronucleus of eac oocyte. In addition, a piece of sperm tail was left near the outer surface of the injected pronucleus.Events that followed IPSHI techniques were : a visible sperm head within the pronucleus ; swelling of the pre-decondensed sperm head within the pronucleus ; an invisible sperm head within the pronucleus ; enlargement of the pronucleus ; and first cleavage in process.Rat IPSHI techniques result in syngamy, mitotic division of zygote. Successful performance of IPSHI techniques may be a novel method to manage cases of ICSI failure due to either a lack of development of the male pronucleus of failure in pronuclei fusion.

卵母细胞活化、精子头在卵质内解凝、雄配子核转化为原核以及原核融合是受精过程的最后步骤。男性和女性配子之间的上述相互作用之一失败会导致 ICSI 失败。我们研究了通过注射预解压缩或预激活卵母细胞来绕过受精过程最后事件的可能性。我们评估了原核内预解压缩精子头注射（IPSHI）后大鼠预激活卵母细胞的发育。成熟大鼠卵母细胞接受电和化学刺激的组合或机械和化学刺激的组合。用IPHSI处理活化的卵母细胞。将预解凝的精子头注射到eac卵母细胞较大的雌性原核中。此外，在注射的原核外表面附近留下了一段精子尾部。IPSHI技术后发生的事件是：原核内可见精子头；原核内预解凝精子头部肿胀；原核内有一个看不见的精子头；原核增大；大鼠IPSHI技术导致合子，受精卵有丝分裂。 IPSHI 技术的成功实施可能是治疗由于雄性原核发育不足或原核融合失败而导致的 ICSI 失败病例的一种新方法。