Studies on establishment of virus-free plants, propagation and acclimatization of garlic and root-promoting treatment of asparagus cultured in vitro.

脱毒植株建立、大蒜繁殖与驯化及离体培养芦笋生根处理的研究。

• 批准号: 61560034
• 负责人: MATSUBARA Sachiko
• 金额: $ 1.09万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61560034/
• 关键词: Establishment of virus-free plant; Apex culture; Dormancy of apex; Acclimatization; Rooting of shoot; flower disc propagation; garlic; ウィルスフリー株の育成; 増殖; 順化; 組織培養; 発根培地; 休眠; えき芽培養; 生長点培養

Establishment of virus-free plants, vegetative propagation and acclimatization in garlic and root-promoting treatment of asparagus plantlets cultured in vitro were studied, and the following results were obtained.Garlic1. Shoot apexes cultured on an initial medium containing 0.01 mg/1 both of NAA and BA and transferred to a subculture medium containing 0.1 mg/1 NAA and 0.01 mg/1 BA grew vigorously and formed bulblets.2. Plantlets grown by the method mentioned above were easily acclimatized on rockwool at 20゜C.3. Virus-free garlic plants were vegetatively propagated by in vitro culture of flower disc cut into 8 explants.Asparagus4. Shoots grown from lateral buds rooted effectively by treatment of Rooton (anaphtylacetoamide 0.4 % in mineral powder) at basal parts. Rooting was also promoted by culturing apexes on MS medium containing 0.3 mg/1NAA and 0.1mg/1 BA for 2-3 weeks and transferring to MA medium.

对大蒜脱毒植株的建立、蔬菜繁殖和驯化以及离体培养的芦笋试管苗的生根处理进行了研究，得到了以下结果。大蒜1． NAA和BA转移到含有0.1mg/1NAA和0.01mg/1BA的继代培养基中生长旺盛并形成2.通过上述方法生长的幼苗很容易在20°C的岩棉上适应。3.通过切成8个外植体的花盘进行体外培养来无性繁殖。通过在基部使用 Rooton（0.4% 萘乙酰胺矿物粉）进行有效处理，可以通过培养顶端来促进生根。在含有 0.3 mg/1NAA 和 0.1mg/1 BA 的 MS 培养基上培养 2-3 周，然后转移至 MA 培养基。