Study on Microcirculation in Islets of Langerhans

朗格汉斯岛微循环的研究

基本信息

批准号：
11671128
负责人：
IWASE Masanori
金额：
$ 2.37万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2002
项目状态：
已结题

项目摘要

1. Measurement of islet blood flow in ratsSince pancreatic islets are widely distributed within the pancreas, a color microsphere method has been adopted for the measurement of islet blood flow. Although a single colored microsphere was used in previous studies, I developed a new method to measure islet blood flow at different time points using different colors of microsphere.2. Islet blood flow in a model of obese type 2 diabetes mellitus, OLETF rats at different agesIslet blood flow was increased by twice in OLETF rats as compared to control, and the increase of islet blood flow was observed at younger age before the development of obesity or hyperglycemia. However, the increase became obscure after worsening hyperglycemia by sucrose feeding, whereas the increase was enhanced after preventing the development of obesity by food restriction. When nitric oxide (NO) synthase inhibitor was administered to young OLETF rats, islet blood flow was no more increased. This suggests that the islet blood hyperperfusion seen in OLETF rats may be mediated by NO-dependent mechanism.3. Effects of cholecystokinin(CCK) on islet blood flowCCK has insulinotropic effect as well as stimulates pancreatic exocrine secretion. CCK enhanced islet blood flow via CCK-A receptors, mediated by NO dependent mechanism, but not by vagal mechanism.4. Effects of nategfinide on islet blood flowNateglinide is a rapid-acting insulin secretagogue, recently introduced to clinical practice. Nateglinide acutely increased islet blood flow, whereas tolbutamide did not have such a effect.5. Islet blood flow in conscious rats and effect of insulin-induced hypoglycemiaI developed a new method to measure islet blood flow in unanesthetized rats. Anesthesia affected blood flow distribution within the pancreas, and vagal pathway prevented the increase of islet blood flow during hypoglycemia.

1。胰岛胰岛中胰岛血流的测量广泛分布在胰腺内，采用了一种颜色的微球方法来测量胰岛血流。尽管在先前的研究中使用了单个彩色微球，但我开发了一种新方法，使用不同颜色的微球在不同时间点测量胰岛流动。2。与对照相比高血糖。然而，在蔗糖喂养加重高血糖后，这种增加变得晦涩难懂，而在防止食物限制肥胖后，增加了增加。当一氧化氮（NO）合成酶抑制剂被施用到年轻的OLETF大鼠中时，胰岛流量不再增加。这表明在OLETF大鼠中看到的胰岛血液过灌注可能是由无依赖性机制介导的。3。胆囊基蛋白（CCK）对胰岛流量CCCK的影响具有胰岛素效应，并刺激胰腺外分泌分泌。 CCK通过CCK-A受体增强的胰岛流动，没有依赖机制介导，但不是通过迷走神经机制介导的。4。 Nategfinide对胰岛血液中的影响是一种快速作用的胰岛素秘密，最近引入了临床实践。 Nateglinide急性增加了胰岛流动，而Tolbutamide则没有这种作用。5。有意识的大鼠的胰岛血流以及胰岛素诱导的低糖菌的作用开发了一种新方法，以测量一体式大鼠的胰岛血流。麻醉影响胰腺内的血流分布，迷走神经途径阻止了低血糖期间胰岛血流的增加。