Studies on ocular toxocariasis in new experimental animal model, Mongolian gerbil

新实验动物模型沙鼠眼弓蛔虫病的研究

• 批准号: 11670240
• 负责人: AKAO Nobuaki
• 金额: $ 2.24万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670240/
• 关键词: Toxocara canis; Toxocara cati; ocular toxocarias; larva migrans; Mongolian gerbil; トキソカラ症

Although Toxocara canis, an important pathogen of zoonosis, tends to migrate to the eye, the precise migratory route has yet to be determined. Mongolian gerbils, Meriones unguiculatus, known as a useful animal model for human toxocariasis, were used to investigate the migration route toward the eyes. Infective larvae of T. canis were directly inoculated into the intracranial region. The hemorrhagic lesions or larvae were observed in 56.3% of cases. Histopathologically, a larva was observed in the optic nerve of gerbils 6 days after inoculation and two larvae were found in the optic chiasma in the gerbils having a hemorrhage in the retina. These results indicate that T. canis migrates from the brain to the eye through the optic nerve. Considering these data and the diversity of the appearance of larvae between the oral-administrated gerbils and the intracranial-injected gerbils, there are two phases in the migration to the retina : a hematogenous early phase and an optic nerve route late phase.

尽管犬弓蛔虫是人畜共患病的重要病原体，它倾向于迁移到眼睛，但精确的迁移路线尚未确定。蒙古沙鼠（Meriones unguiculatus）被称为人类弓蛔虫病的有用动物模型，被用来研究向眼睛的迁移路线。将感染性犬毛虫幼虫直接接种到颅内区域。 56.3%的病例出现出血性病变或幼虫。组织病理学观察，接种后6天，在沙鼠视神经中观察到1条幼虫，在视网膜出血的沙鼠视交叉中发现2条幼虫。这些结果表明，犬毛虫通过视神经从大脑迁移到眼睛。考虑到这些数据以及口服沙鼠和颅内注射沙鼠幼虫外观的多样性，向视网膜的迁移有两个阶段：血行早期阶段和视神经途径晚期阶段。

项目成果

本橋尚子: "ブラジル鉤虫によると思われたcreeping eruptionの1例"皮膚科の臨牀. (印刷中). (2002)

Naoko Motohashi：“一例被认为是由巴西钩虫引起的蠕动性皮疹”，皮肤科诊所（2002 年出版）。

赤尾信明: "回虫症(イヌ・ネコ回虫症を含む)"小児科臨床. 52・4. 270-277 (1999)

赤尾伸明：“蛔虫病（包括犬和猫蛔虫病）”儿科临床 52・4 270-277（1999）。

Akao N: "Helminthology(分担)Serological diagnosis of Nematode infection"Narosa(in press). (2002)

Akao N：“线虫感染的蠕虫学血清学诊断”Narosa（出版中）（2002 年）。

Takayanagi T et al.: "New animal model for human ocular toxocariasis : Ophthalmoscopic observation"British. Journal of Ophthalmology. 83. 967-972 (1999)

Takayanagi T 等人：“人类眼弓蛔虫病的新动物模型：检眼镜观察”英国。

赤尾信明: "皮膚疾患をおこす虫と海生動物の図鑑 寄生虫"皮膚病診療. 22. 112-125 (2000)

Nobuaki Akao：“引起皮肤病的昆虫和海洋动物图解百科全书：寄生虫”皮肤病学治疗。