Study on the role of nitric oxide on sweating

一氧化氮对出汗作用的研究

• 批准号: 11670077
• 负责人: MATSUMOTO Takaaki
• 金额: $ 2.3万
• 依托单位: Aichi Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670077/
• 关键词: cat paw sweating; skin vasodilation; acetylcholine; pilocarpine; tibial nerve stimulation; videomicroscope; axon-reflex sweating; cat paw sweating; skin vasodilation; acetylcholine; pilocarpine; tibial nerve stimulation; videomicroscope; axon-reflex sweating

Reflex control of skin blood flow in human is accomplished through sympathetic vasoconstrictor nerves and a sympathetic active vasodilator system. Cutaneous active vasodilation is closely related to the activation of sweat glands and is suggested to be mediated by peptidergic cotransmitters, such as VIP or CGRP, of the cholinergic sympathetic nerve. However, the mechanisms of active vasodilator system is not fully understood, because of the lack of the animal model. Therefore, we tried to develop a cat paw model. In 4 anesthetized cats, sweating on the paw pad was measured by a ventilated capsule method and skin blood flow was measured with a laser-Doppler flowmeter. Spontaneous sweating was disappeared after anesthesia. Local and systemic administration of accetylcholine (ACh) and pilocarpine induced sweating without cutaneous vasodilation. Electric stimulation of the tibial nerve induced sweating with cutaneous vasodilation on the hind paw. Video-microscope (× 25-175) enabled us to o … More bserve sweat emergence from the gland pores, and showed the sweat gland density of about 13-15 per mm^2. These results indicate that cat paw is a useful animal model to study the mechanisms of cutaneous active vasodilation. In the human subjects, on the ventral surface of the forearm nicotine (10^<-4> g/ml, ip) was administered, and sweat rate and cutaneous blood flow were measured at the sites beyond a rubber band bound around the arm. Nicotine induced axone-reflex sweating as well as skin vasodilatation, and the both responses parallely changed throughout observation period. The previously administered atropine blocked the sweating response induced by nicotine, but failed to block the cutaneous vasodilatation on the same area. The results indicate that skin vasodilatation accompanied with axone-reflex sweating is probably mediated, not by muscarinic action of a ACh, but by some vasodilative polypeptides, such as VIP or CGRP co-released with ACh from the postganglionic sudomotor nerve terminals. Further understanding is expected through the pharmaceutical approaches using selective blockers of these vasoactive peptide in the useful cat paw sweating model. Less

通过交感神经的神经和交感神经活性血管扩张系统来实现人类皮肤血流的反射控制。皮肤活性血管舒张与汗腺的激活密切相关，并建议由胆碱能交感神经系统的Pepperigic共透明剂（例如VIP或CGRP）介导。但是，由于缺乏动物模型，因此尚未完全了解活性血管扩张系统的机制。因此，我们试图开发猫爪模型。在4个麻醉猫中，通过通风胶囊方法测量爪垫上的出汗，并用激光多普勒流量计测量皮肤血​​流。麻醉后自发出汗消失了。局部和全身给药加速胆碱（ACH）和毛car骨诱导出汗而无皮肤血管舒张。胫骨神经诱导出汗并在后爪上刺激出汗。视频微观显微镜（×25-175）使我们能够……从腺体毛孔中出现汗水，并显示出每毫米约13-15的汗腺密度。这些结果表明，猫爪是研究皮肤活性血管舒张机制的有用动物模型。在人类受试者中，对前臂尼古丁（10^<-4> g/ml，ip）的腹表面进行了，并在手臂周围围绕的橡皮筋之外的位置测量了汗液和皮肤血流。尼古丁诱导轴突反射出汗以及皮肤血管舒张，并且在整个观察期间，两种反应都平行。先前给药的阿托品阻止了尼古丁引起的出汗反应，但未能阻止同一区域的皮肤血管扩张。结果表明，伴有轴突反射出汗的皮肤血管舒张可能是介导的，不是通过ACH的毒蕈碱作用介导，而是通过某些血管舒张多肽的作用，例如VIP或CGRP与ACH共同发行的ACH，从后旋转后的Sudomotor Nerver终端进行了ACH。通过使用这些血管活性肽的选择性阻滞剂在有用的CAT PAW出汗模型中使用这些血管活性肽的选择性阻滞剂，可以预期进一步理解。较少的