Development of Whole Cell-Immobilized Biosensors Using Electron Transfer Mediator for Detection of Pollutants in Environment

利用电子转移介体开发全细胞固定化生物传感器来检测环境中的污染物

基本信息

批准号：
11660331
负责人：
MIKI Kojiro
金额：
$ 1.47万
依托单位：
Nara National College of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11660331/
关键词：
Bioelectrocatalysis Biosensor Whole Cell Electron Transfer Mediator Cyanide Herbicide

项目摘要

Whole cell modified electrode using Baker's yeast cells produced an anodic current approaching a steady state with the addition of electron transfer mediator. When glucose was further added to the solution, the anodic current started to increase again. The current magnitude depends on the concentration of NADH in the cells. The anodic current with glucose increased when yeast cells at logarithmic growth phase were used. The current increased with the addition of KCN due to an increase in the concentration of intracellular NADH.Thus, the electrode employing whole-cells of baker's yeast may be used for the determination of number of cells and the detection of cytotoxic compoundsAn electrode with immobilized mycelium of Aspergillus niger also produced an anodic current with the addition of mediators. The scnsitivity of the current response with carbohydrates was influenced by the cultivation medium in which A.niger was cultured.An electrode with immobilized Chorella vulgaris Beijerinck cell also produced an anodic current with a white light illumination in the presence of p-benzoquinone in a solution. The anodic current is due to the photosynthesis of C.vulgaris. Since a herbicide such as Diuron inhibits the photosynthesis of C.vulgaris, it may be detected by the decrease in the currentGlycolytic oscillation of a metabolite has been successfully monitored with a bioelectrochemical sensor for pyruvate, and the significance of the NAD^+/NADH ratio in the glycolytic oscillation is proposed.

使用贝克酵母细胞的全细胞修饰电极通过添加电子转移介质产生了接近稳态的阳极电流。当葡萄糖进一步添加到溶液中时，阳极电流再次开始增加。当前的幅度取决于细胞中NADH的浓度。当使用对数生长阶段的酵母细胞时，具有葡萄糖的阳极电流增加。由于细胞内NADH的浓度升高，使用KCN的电流增加，因此，使用贝克酵母全细胞的电极可用于确定细胞数量，并且检测细胞毒性化合物电极与氧化菌Niger的固定菌丝的菌丝菌丝还产生了阳离子的阳离子，也与阳离子的速度产生了阳极氧化剂的当前。当前对碳水化合物的反应的SCN呈率受培养的培养培养基的影响。尼日的培养介质具有固定的抗硫酸脉络膜北京Chorella bulgaris beijerinck细胞的电极也产生了阳极电流，在溶液中存在p-benzoquinone的情况下，具有白光照明。阳极电流是由于C.Vulgaris的光合作用引起的。由于除草剂（例如硫龙）抑制了c.vulgaris的光合作用，因此可以通过生物电化学传感器成功地监测了代谢物的当前当前糖酵解振荡的检测，而丙酮酸的生物化学传感器已成功地监测了NAD^+/NADH比率的意义。