Study on Influence of Zeta-Potentials of Solute and Membrane on Electro-Ultrafi Itration of Protein Solutions

溶质和膜的Zeta电位对蛋白质溶液电超滤的影响研究

基本信息

批准号：
11650802
负责人：
YOSHIDA Hiroshi
金额：
$ 1.22万
依托单位：
Oyama National Colleg of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11650802/
关键词：
Electro-Ultrafi Itration Ultrafi Itration Membrane Protein Solution Zeta-Potential Electric Power Appl ications Filtration Cake Resistance Permeate Flux Solute Rejection 限外濾過流速ゼーダ電位

项目摘要

1. Bovine serumalbumin (BSA) and haemoglobin were used as the experimental material for protein solution.They have almost the same molecular weight (MW) but isoelectric points (IP) of them, in which zeta-potential representing surface electric charge of protein is zero, were different : MW=67,000, IP=4.8 for BSA, and MW=68,000.IP=6.8 for haemoglobin. For ultrafiItration membrane, a polymeric membrane of which MW cut off is 50,000 was used. The zeta-potentials of proteins and membrane were investigated with varying pH of protein solution and liquid permeating through the membrane. UltrafiItaration and electro-ultrafiItration were discussed on the basis of the zeta-potential data.2. Using a dead-end filtration experimental apparatus, ultrafiItration was performed experimentally by varying pH of protein solution, and the flow rate for cake filtration, membrane permeate flux and solute rejection were investigated. The filtration characteristics were almost related with the zeta-potential o … More f protein only : the filtration rate and the permeate flux were the smallest in the IP of each protein solution and were nearly the same in the IPs in both prot-Bin solutions, and they were increased with increasing the absolute value of zeta-potential irrespective of the polarity of it. The process of ultra-filtrationwashardly in fluenced by the zeta-potential of membrane.3. An experimental apparatus for electro-ultrafiItration was made, and the process of electroultrafiItration of BSA solution was investigated under the operation of constant voltage applied. Under D.C. electric field, the fiItration rate was increasedwith increasing the voltage, because electro-phoresis caused by the zeta-potential of protein was enhanced under higher electric field, but there was no electrical effect in the IP. In the rangeofexperimental conditions used, under A.C. field of whichthe polarityof electrodes is reversed, thefiItration ratewas recoveredperiodicallybutsiightlydecreasedwiththetime. Intermittent power application also was not available for improving electro-ultrafiItration. Less

1。牛柔毛（BSA）和血红蛋白用作蛋白质溶液的实验材料。它们具有几乎相同的分子量（MW），但是它们的等电点（IP），其中Zeta代表蛋白质的表面电荷为零，不同：MW = 67,000，BSA的IP = 4.8，MW = 68,000，血红蛋白的IP = 6.8。对于超铁膜，使用了50,000的MW切断的聚合物膜。用蛋白质溶液的不同pH和液体渗透到膜中，研究了蛋白质和膜的Zeta势率。根据Zeta-PotitalDation的数据，讨论了超级武器和电粉末化。2。使用死端过滤实验设备，通过不同的蛋白质溶液的pH来实验进行超级武器，并研究了蛋糕过滤，膜渗透通量和固体排斥的流速。过滤特性几乎与Zeta竞争性O…仅相关。仅F蛋白更多：过滤速率和渗透通量是每种蛋白质溶液的IP中最小的，并且在两种Prot-BIN溶液中IPS中几乎相同，并且随着Zeta-Patiental的绝对值而增加，并且它们的极值的绝对价值不断增加。在Zeta-potential Membrane中流动的超滤过的过程3。进行了用于电 - 脱脂二烯的实验设备，并在施加恒定电压的运行下研究了BSA溶液的电 - 硫酸溶液的过程。在哥伦比亚特区电场下，由于电压的增加，由于蛋白质Zeta势率引起的电玻璃孔增加了电压的增加，因此在较高的电场下增加了电孔，但是IP中没有电效应。在所使用的范围内的条件下，在电极的极性磁极下，fiitration rate ratewas恢复了periodyperiplydersightlydecrastered withtheTime。间歇性的功率应用也无法改善电 - 脱脂率。较少的