The study for origin and evolution of biosynthesis of chlorophyll α

叶绿素α生物合成的起源与演化研究

• 批准号: 11640657
• 负责人: INOUE Kazuhito
• 金额: $ 2.11万
• 依托单位: Kanagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11640657/
• 关键词: photosynthesis; chlorophyll; evolution; chloroplasts; photosynthetic bacteria; cyanobacteria; バクテリオクロロフィル; 色素合成

To elucidate the origin and evolution of biosynthesis of chlorophyll α, the following study have been done.1. Cloning of genes which involved in bacteriochlorophyll biosynthesis from A cidiphilium rubrum which produce Zn-bacteriochlorophyll.2. Cloning of genes which involved in bacteriochlorophyll biosynthesis from green non-sulfur bacterium Chloroflexus aurantiacus.3. Cloning of genes which involved in bacteriochlorophyll biosynthesis from green sulfur bacterium Chlorobium tepidum.4. Phylogenetic analyses of multi magnesium-tetrapyrrole biosynthesis genes indicates bacteriochlorophyll biosynthesis evolved before chlorophyll biosynthesis.

为了阐明叶绿素α的生物合成的起源和进化，已经完成了以下研究。1。从cidiphilium rubrum中涉及细菌氯粘合菌生物合成的基因的克隆，该基因产生了Zn-细菌氯酚2。从绿色非硫细菌aurantiacus的绿色非硫细菌中涉及细菌氯粘合菌生物合成的基因克隆。3。从绿硫细菌tepidum.4中涉及细菌氯叶藻生物合成的基因克隆。4。多镁四吡咯生物合成基因的系统发育分析表明叶绿素生物合成在叶绿素生物合成之前演变。

项目成果

Xiong J., Fischer W.M., Inoue K., Nakahara M.and Bauter C.E.: "Molecular evidence for the early evolution of photosynthesis."Science. 289. 1724-1730 (2000)

Xiong J.、Fischer W.M.、Inoue K.、Nakahara M. 和 Bauter C.E.：“光合作用早期进化的分子证据。”科学。

Masuda T., Inoue K., Masuda M., Nagayama M., Tamaki A., Ohta H., Shimada H.and A.Takamiya.: "Magnesium insertion by magnesium chelatase in the biosynthesis of zinc-bacteriochlorophyll a in an aerobic acidophilic bacterium Acidiphilium rubrum."J.Biol.Chem.

Masuda T.、Inoue K.、Masuda M.、Nagayama M.、Tamaki A.、Ohta H.、Shimada H. 和 A.Takamiya.：“有氧细菌中锌-细菌叶绿素 a 生物合成中镁螯合酶插入镁

Masuda T.,Inoue K. et al.: "Magnesium insertion by magnesium chelatase in the biosynthesis of zinc-bacteriochlorophyll a in an aerobic acidophilic bacterium Acidiphilium rubrum."J.Biol.Chem.. 274. 33594-33600 (1999)

Masuda T.，Inoue K.等人：“在需氧嗜酸性细菌 Acidiphilium rubrum 中锌细菌叶绿素 a 生物合成中镁螯合酶插入镁。”J.Biol.Chem. 274. 33594-33600 (1999)

Masuda S., (他6名): "Structural and functional analysisi of photosynthetic regulatory genes, regA and regB from Rhodovulum sulfidophilum, Roseobacter denitrificans and Rhodobacter capsulatus."Journal of bacteriology. 181,14. 4205-4215 (1999)

Masuda S.，（其他 6 名）：“来自嗜硫红酵母、脱氮玫瑰杆菌和荚膜红杆菌的光合调节基因 regA 和 regB 的结构和功能分析。”细菌学杂志 181,14（1999）。

Xiong J.,Fischer W.M.,Inoue K.,Nakahara M.and Bauer C.E.: "Molecular evidence for the early evolution of photosynthesis."Science. 289. 1724-1730 (2000)

Xiong J.，Fischer W.M.，Inoue K.，Nakahara M.和 Bauer C.E.：“光合作用早期进化的分子证据。”科学。