Effect of Magnetic Field on Stage of Cell Division

磁场对细胞分裂阶段的影响

基本信息

批准号：
10837016
负责人：
SAITO Masao
金额：
$ 1.79万
依托单位：
Tokyo Denki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

We examined the effectrs of the magnetic field in terms of the mitochondria activity, the cell respiration, the cell morphogenesis, the cell adhesions and intercellular or extracellular of the ion density.1. Effects of the magnetic field on the mitochondria activityAs a preliminary examination, the time required to render the cells to the low energy state, I.e., to the G0/G1 phase of the cell cycle, and to reduce the metabolic activity level, was examined by keeping the cells in the medium with low-density fetal bovine serum. We examined the effect of the magnetic field on the metabolic activity of the mitochondrial by estimating the cell metabolism using the MTT assay. The magnetic field was kept at 100[mT]as well as 0[mT]for the control. The results by MTT assay indicated that the metabolic activity of the mitochondria seems to been enhanced by the factor of 1.5.2. Effects of the magnetic field on the cell respirationThe metabolic activity of the mitochondria is considered to be refl … More ected on its respiration. The cell respiration is increased by the factor of 1.3 by the exposed to the magnetic field. In other words, the change of the cell respiration by the magnetic field is very small, as seen from the change of the oxygen in the medium.3. Effects of the magnetic field on the intracellular and extracellular of ion densityWe concern with the calcium ion transport between the intracellular and the extracellular fluid, in order to examine this aspect. We examined the change of ATP when exposed to the magnetic field. When ATP is added to the cell, the cell metabolic activity is enhanced. While, exposed to the magnetic field, even if, ATP is added, the activity is not enhanced.4. Effects of the magnetic field on the cell morphogenesis and cell adhesionThe cell morphogenesis is observed by imaging by using a phase-sensitive microscope and CCD camera. When exposed to the magnetic field, the morphogenesis development is inhibited by approx. 40% than that of the control. In order to analyze the factor for inhibition, we examined the cell adhesion by the crystal violet assay. It is indicated that, when exposed to the magnetic field, the cell adhesion is inhibited by approx. 30% than that of the control. Less

我们根据线粒体活性，细胞呼吸，细胞形态发生，细胞粘附以及离子密度的细胞间或细胞细胞的影响，检查了磁场的影响。1。磁场对线粒体活性的影响是初步检查，即使细胞到低能量状态，即进入细胞周期的G0/G1阶段所需的时间，并降低代谢活性水平，通过将细胞保持在低密度胎牛血清中的培养基中进行检查。我们通过使用MTT测定法估算细胞代谢，研究了磁场对线粒体代谢活性的影响。对照将磁场保持在100 [mt]和0 [mt]。 MTT测定法的结果表明，线粒体的代谢活性似乎已增强了1.5.2因子。磁场对细胞呼吸的影响，线粒体的代谢活性被认为是反射的……在其呼吸方面更受欢迎。暴露于磁场的情况下，细胞呼吸增加了1.3。换句话说，从介质中的氧气变化可以看出，磁场通过磁场呼吸的变化很小。3。磁场对细胞内和细胞外离子密度的影响关注细胞内和细胞外液之间的钙离子转运，以检查这一方面。我们检查了暴露于磁场时ATP的变化。当将ATP添加到细胞中时，细胞代谢活性会增强。而，即使添加了ATP，也不会增强活动。4。磁场对细胞形态发生和细胞粘附的影响，通过使用相敏感显微镜和CCD摄像机进行成像观察细胞形态发生。当暴露于磁场时，形态发生的发展受到大约的抑制。 40％比控制。为了分析抑制因素，我们通过晶体紫色测定法检查了细胞粘合剂。据表明，当暴露于磁场时，细胞粘合剂被大约抑制。 30％比控制。较少的