Effects of Heavy-Ion Beam Irradiation on Histochemical and Enzymatic Properties of Skeletal Muscles and Bone Metabolism in Young and Old SHRSP Rats.

重离子束照射对年轻和老年 SHRSP 大鼠骨骼肌组织化学和酶学特性以及骨代谢的影响。

基本信息

批准号：
10680514
负责人：
TAGUCHI Sadayoshi
金额：
$ 0.58万
依托单位：
KYOTOUNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10680514/
关键词：
Heavy ion irradiation Stroke prone spontaneously hypertensive rat Enzymaticactivity Bone metabolism

项目摘要

The present study was designed to determine whether heavy-ion beam irradiation is associated with histochemicalproperties in the skeletal muscle and bone metabolismin young and old SHRSP (stroke prone spontaneously hypertensive rat) rats. For this objective, we carried out following four experiments, 1998〜1999; 1) Effect of heavy-ion irradiation on histochemicalproperty of muscle fibers in young SHRSP rats, 2) Comparative study of histochemicalproperties in skeletal muscle fibers between young and old Wistar rats, 3) Effect of calcium-supplementaionon the myosin heavy chain expression of skeletal muscles in SHR rats following spaceflight, 4) Effects of X-ray irradiation during unweighting on histochemicalproperties of skeletal muscles in rats.Generally, the highly heavy-ion irradiated group morphometrically displayed a significant alteration in fiber type distribution with an increase of intermediate fibers, and atrophy of SO fibers while those exhibiting little or no change were in ox … More idative potential. Irradiated groups, especially three-month-old groups had a tendency to decrease in alpha-gycerophosphate dehydrogenase activity in individual fibers. And, the fermur strength was not changed after heavu-ion irradiation. In third exp., in the soleus muscle, both groups of spaceflightrats regardless of Ca intake increased FOG type fibers (24%) as compared to ground-control. Electrophoretic analysis exhibited higher composition in the type IIx MHC (24〜28%) of the soleus muscles in both spaceflight groups of rats while the ground control rats had 0% type IIx fibers. There was a decrease in type I MHC in both spaceflight groups of rats. The changes in MHC isoforms suggest that microgravity produced the transitionof slow to fast twitch fibers in antigravitymuscles regardless of differences in dietary calcium. Fourth exp. examined that SC and SX groups, after 2 weeks of suspension, an increase in the percentage of intermediatefibers was counterbalancedby a simultaneous significantdecrease in the SO fibers. The SDH activity in the SO fibers in SC and SX rats was significantly increased compared to both of ground groups as well as seen in the α-GPD activity. These findings indicate that the microgravity was more morphometrically and metabolicallyeffective in the muscles than X-ray irradiation. Less

本研究旨在确定重型离子束辐照是否与骨骼肌中的组织化学特质和骨骼代谢中的组织化学特质有关，而骨骼代谢的年轻人和老shRSP（中风易于赞助高血压大鼠）大鼠。为了实现这一目标，我们在1998 - 1999年进行了四个实验之后进行了。 1）重离子辐照对年轻SHRSP大鼠组织化学特性的影响，2）对年轻人和老年大鼠之间骨骼肌纤维中的组织化学性质的比较研究，3）钙 - 供应钙的影响，钙 - 辅助性的效果大鼠中的骨骼肌。从总体上讲，高度重的辐照组形态图表显示出纤维类型分布的显着变化，中间纤维的增加，而如此纤维的萎缩却很少或没有变化，而在OX中表现出很小的变化是……具有更大的身份潜力。辐照组，尤其是三个月大的组在单个纤维中α-甲磷酸酯脱氢酶活性的趋势趋于降低。而且，在重型离子辐照后没有改变费列的强度。在比目鱼肌中，在第三次经验中，与地面控制相比，两组太空浮游剂都增加了雾型纤维（24％）。电泳分析暴露于两种大鼠飞行组的iix MHC型（24-28％）中的较高组成，而地面控制大鼠具有0％型IIX型纤维。在两种太空大鼠的飞行组中，I型MHC均下降。 MHC同工型的变化表明，微重力型在抗古ravitymuscles中产生慢速到快速抽搐纤维的过渡，而不管饮食钙的差异如何。第四个经验。检查了SC和SX组在悬浮2周后，中间纤维的百分比增加是SO纤维的简单显着降低。与两者组相比，SC和SX大鼠的SO纤维中的SDH活性显着增加，并且在α-GPD活性中所见。这些发现表明，与X射线照射相比，微重力在肌肉中更有和代谢上有效。较少的