Effects of Heavy-Ion Beam Irradiation on Histochemical and Enzymatic Properties of Skeletal Muscles and Bone Metabolism in Young and Old SHRSP Rats.

重离子束照射对年轻和老年 SHRSP 大鼠骨骼肌组织化学和酶学特性以及骨代谢的影响。

基本信息

批准号：
10680514
负责人：
TAGUCHI Sadayoshi
金额：
$ 0.58万
依托单位：
KYOTOUNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10680514/
关键词：
Heavy ion irradiation Stroke prone spontaneously hypertensive rat Enzymaticactivity Bone metabolism

项目摘要

The present study was designed to determine whether heavy-ion beam irradiation is associated with histochemicalproperties in the skeletal muscle and bone metabolismin young and old SHRSP (stroke prone spontaneously hypertensive rat) rats. For this objective, we carried out following four experiments, 1998〜1999; 1) Effect of heavy-ion irradiation on histochemicalproperty of muscle fibers in young SHRSP rats, 2) Comparative study of histochemicalproperties in skeletal muscle fibers between young and old Wistar rats, 3) Effect of calcium-supplementaionon the myosin heavy chain expression of skeletal muscles in SHR rats following spaceflight, 4) Effects of X-ray irradiation during unweighting on histochemicalproperties of skeletal muscles in rats.Generally, the highly heavy-ion irradiated group morphometrically displayed a significant alteration in fiber type distribution with an increase of intermediate fibers, and atrophy of SO fibers while those exhibiting little or no change were in ox … More idative potential. Irradiated groups, especially three-month-old groups had a tendency to decrease in alpha-gycerophosphate dehydrogenase activity in individual fibers. And, the fermur strength was not changed after heavu-ion irradiation. In third exp., in the soleus muscle, both groups of spaceflightrats regardless of Ca intake increased FOG type fibers (24%) as compared to ground-control. Electrophoretic analysis exhibited higher composition in the type IIx MHC (24〜28%) of the soleus muscles in both spaceflight groups of rats while the ground control rats had 0% type IIx fibers. There was a decrease in type I MHC in both spaceflight groups of rats. The changes in MHC isoforms suggest that microgravity produced the transitionof slow to fast twitch fibers in antigravitymuscles regardless of differences in dietary calcium. Fourth exp. examined that SC and SX groups, after 2 weeks of suspension, an increase in the percentage of intermediatefibers was counterbalancedby a simultaneous significantdecrease in the SO fibers. The SDH activity in the SO fibers in SC and SX rats was significantly increased compared to both of ground groups as well as seen in the α-GPD activity. These findings indicate that the microgravity was more morphometrically and metabolicallyeffective in the muscles than X-ray irradiation. Less

本研究旨在确定重离子束照射是否与年轻和老年 SHRSP（易发生中风的自发性高血压大鼠）大鼠的骨骼肌和骨代谢的组织化学特性相关。为此，我们进行了以下四项实验： 1998〜1999；1）重离子照射对幼年SHRSP大鼠肌纤维组织化学性质的影响，2）比较研究年轻和老年Wistar大鼠骨骼肌纤维的组织化学特性，3）补钙对航天后SHR大鼠骨骼肌肌球蛋白重链表达的影响，4）减重过程中X射线照射对大鼠骨骼肌组织化学特性的影响一般来说，高重离子照射组在形态上表现出纤维类型分布的显着变化，随着纤维类型分布的增加中间纤维和SO纤维萎缩，而那些表现出很少或没有变化的群体，尤其是三个月大的群体，单个纤维中的α-甘油磷酸脱氢酶活性有下降的趋势，在第三次实验中，在重离子照射后，两组太空飞行大鼠的比目鱼肌中的股骨强度没有变化，无论钙摄入量如何，FOG 型纤维都会增加。电泳分析显示，两个航天组大鼠的比目鱼肌的 IIx 型 MHC 成分（24%）较高，而地面对照大鼠的 IIx 型纤维含量为 0%。两组太空飞行大鼠的 I 型 MHC 均有所减少。MHC 异构体的变化表明，无论差异如何，微重力都会导致抗重力肌肉中慢肌纤维向快肌纤维的转变。第四次实验检查了 SC 和 SX 组，停药 2 周后，中间纤维百分比的增加被 SO 纤维的同时显着降低所抵消。与地面组相比，α-GPD 活性显着增加。这些发现表明，微重力在肌肉形态和代谢方面比 X 射线照射更有效。较少的