Relevance Between Apoptosis And Ratio of Bax/Bcl-2 in Cancer Cells

癌细胞凋亡与Bax/Bcl-2比值的相关性

• 批准号: 10672185
• 负责人: KAETSU Sachiyo
• 金额: $ 1.73万
• 依托单位: SHOWA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10672185/
• 关键词: Bcl-2; Bcl-XL; Bax; APOPTOSIS; p53; Bad; PKB; AKT; caspase 3; cisplatin; caspase; CDDP

Bax and Bad is known as proapoptotic protein. When they are heterodimerized with Bcl-2 or Bcl-XL which s known as anti-apoptotic protein, apoptosis is proceed. But when cells are receaved with some stress of proliferation, Bax and Bad are separated and Bcl-2/Bcl-XL are made anti-apoptotic homodimerizons, cells are proliferated. Our study 1) detection of Bcl-2 family protein and mRNA, p53 protein and caspase 3, 2) When we use anti-cancer drugs, we analuzed Bcl-2 family protein using cell line.

Bax 和 Bad 被称为促凋亡蛋白。当它们与抗凋亡蛋白Bcl-2或Bcl-XL异二聚化时，细胞凋亡就会进行。但当细胞受到一定的增殖应激时，Bax和Bad分离，Bcl-2/Bcl-XL形成抗凋亡同源二聚体，细胞增殖。我们的研究1）Bcl-2家族蛋白和mRNA、p53蛋白和caspase 3的检测，2）当我们使用抗癌药物时，我们利用细胞系分析了Bcl-2家族蛋白。

项目成果

K. Fukuchi, et al.: "DNA damage induces p21 protein expression by inhibiting ubiquitination in ML-1 cells"Biochimica et Biophysica Acta. 1404. 405-411 (1998)

K. Fukuchi 等人：“DNA 损伤通过抑制 ML-1 细胞中的泛素化来诱导 p21 蛋白表达”Biochimica et Biophysica Acta。

K.Fukuchi, et al.: "Direct proteasome inhibition by clastolactacyastin β-lactone permits the detection of ubiquitinated p21^<waf1> in ML-1 cells."Biochimica et Biophysica Acta. 1451. 206-210 (1999)

K. Fukuchi 等人：“通过 clastolactacyastin β-内酯直接抑制蛋白酶体，可以检测 ML-1 细胞中泛素化的 p21^<waf1>。”Biochimica et Biophysica Acta. 1451. 206-210 (1999)

K.Fukuchi,et al.: "DNA damage induces p21 protein expression by inhibiting ubiquitination in ML-1 cells" Biochimica et Biophysica Acta. 1404. 405-411 (1998)

K.Fukuchi 等人：“DNA 损伤通过抑制 ML-1 细胞中的泛素化来诱导 p21 蛋白表达”Biochimica et Biophysicala Acta。

K.Fukuchi, et al.: "DNA damage induces p21 protein expression by inhibiting ubiquitination in ML-1 cells"Biochimica et Biophysica Acta. 1404. 405-411 (1998)

K.Fukuchi 等人：“DNA 损伤通过抑制 ML-1 细胞中的泛素化来诱导 p21 蛋白表达”Biochimica et Biophysicala Acta。