Fas antigen and Fas ligand with Oral Disease

Fas抗原和Fas配体与口腔疾病

• 批准号: 10671900
• 负责人: FUKUDA Jinichi
• 金额: $ 1.98万
• 依托单位: Kyushu Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671900/
• 关键词: Fas antigen; Fas ligand; apoptosis; oral cancer

To determine the relationship between Fas antigen and apoptosis in oral epithelium, we investigated the expression of Fas antigen in oral mucosa by immunohistochemical and immunoblotting methods.Fas antigen distributed in stratum spinosum and basal part of the stratum coreneum in normal human gingiva. The estimated molecular weight of Fas antigen was calculated as 35,000, which consistent with the value reported for the Fas antigen. Expression of Fas antigen was related to the degree of the tumor differentiation.Using RT-PCR, messenger RNA for Fas antigen was detected in the human oral squamous cell carcinoma cell line, SCC-25. In serum-free medium, a monoclonal ante-Fas antibody induced expression of Fas antigen in SCC-25 cells. Fas antigen was localized to the cytoplasm and the cell membrane. The molecular weight of the protein recognized was 35,000. Anti-Fas monoclonal antibody induced cytotoxicity in SCC-25 cells in a time-dependent manner up to 8 h. Marked nuclear condensation and fragmentation of chromatin were observed in the anti-Fas monoclonal antibody-treated cells using Hoechst 33342 staining. This antibody also induced DNA ladder formation in SCC-25 cells in a time-dependent manner. The present results indicate that the anti Fas monoclonal antibody may mediate apoptosis by binding to the Fas antigen expressed in SCC-cells.

为了确定口服上皮中FAS抗原与凋亡之间的关系，我们通过免疫组织化学和免疫印迹方法研究了口服粘膜中Fas抗原的表达。FAS抗原分布在脊柱脊柱中，在正常人类gingiva中分布在脊柱的基础和基础。 FAS抗原的估计分子量计算为35,000，这与FAS抗原报告的值一致。 Fas抗原的表达与肿瘤分化的程度有关。使用RT-PCR，在人口服鳞状细胞癌细胞系SCC-25中检测到FAS抗原的Messenger RNA。在无血清培养基中，单克隆ante-Fas抗体在SCC-25细胞中诱导的Fas抗原表达。 FAS抗原位于细胞质和细胞膜。识别的蛋白质的分子量为35,000。抗FAS单克隆抗体在SCC-25细胞中以时间依赖的方式诱导细胞毒性。使用HOECHST 33342染色观察到在抗FAS单克隆抗体处理的细胞中观察到染色质的明显核凝结和碎片化。该抗体还以时间依赖的方式在SCC-25细胞中诱导DNA梯子形成。目前的结果表明，抗FAS单克隆抗体可以通过与SCC细胞中表达的FAS抗原结合来介导凋亡。

项目成果

Fujita, M.: "Induction of apoptosis in human oral squamous carcinoma cell lines by protein phsphatase inhibitors"European Journal of Cancer Oral Onoology. 35. 401-408 (1999)

Fujita, M.：“蛋白磷酸酶抑制剂诱导人口腔鳞状癌细胞系凋亡”欧洲癌症口腔肿瘤学杂志。

Morimoto, Y.: "The protein phosphatase inhibitors, okadaic acid and calyculin A, induce apoptosis in human submandibular gland ductal cell line HSG cells"Oral Diseases. 5. 10-110 (1999)

Morimoto, Y.：“蛋白磷酸酶抑制剂冈田酸和花萼蛋白 A 可诱导人颌下腺导管细胞系 HSG 细胞凋亡”口腔疾病。

Muraki Y., Yoshioka C., Tateishi A., Fukuda J., Haneji T.and Kobayashi N: "Localization of Fas antigen in oral squamous cell carcinoma"Brit. J. Oral Maxillofac. Surg. 37. 37-40 (1999)

Muraki Y.、Yoshioka C.、Tateishi A.、Fukuda J.、Haneji T. 和 Kobayashi N：“Fas 抗原在口腔鳞状细胞癌中的定位”Brit。

Muraki,Y.: "The significance of PCNA lebeling index at tumor invasion front in oral squamous cell carcinomas"The Asian Journal of Oral and Maxillofacial Surgery. 11. 41-46 (1999)

Muraki,Y.：“口腔鳞状细胞癌肿瘤侵袭前沿的 PCNA 标记指数的意义”亚洲口腔颌面外科杂志。

Yasuhiro Morimoto: "The protein phosphatase inhibitors, okadaic acid and calyculin A induce apoptosis in human submandibular gland cell line HSG cells" Oral Diseases. in press. (1999)

Yasuhiro Morimoto：“蛋白磷酸酶抑制剂、冈田酸和花萼蛋白 A 诱导人颌下腺细胞系 HSG 细胞凋亡”口腔疾病。