Role of macrophages on periodontal destruction in rat experimental periodontitis caused by topical application of lipopolysaccharide

巨噬细胞在局部应用脂多糖所致大鼠实验性牙周炎牙周破坏中的作用

基本信息

批准号：
10671698
负责人：
IJUHIN Naokuni
金额：
$ 2.18万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

In this research, we tried to elucidate experimentally a role of macrophage/monocyte system on inflammatory changes in marginal periodontal tissue caused by lipopolysaccharide (LPS). The LPS from E. coli were administered once and twice from the molar gingival sulcus of a male Wistar rats, and the dynamics of macrophages in the rat marginal periodontal tissue, synthesis of the cytokines such as IL-1 β and dynamics of the osteoclasts and lymphocytes were investigated using an immunohistochemical method. As this research was done succeeding from the previous research projects, a part. of the results were reported again with the newly obtained data. As a result, 1. After once topical application of LPS, it was revealed that ED1 positive macrophages and Ia positive macrophages increased especially in the subepithelial connective tissue of the gingiva. It was suggested that some of these macrophages might produce IL-1β and activate osteoclastic alveolar bone resorption. 2. Twice application … More of LPS caused remarkable increase of ED1 positive cells and Ia positive cells in an early stage. It was suspected that these Ia positive cells might participate establishment of specific immune response in the lesion. 3. Although Pan T and pan B cells were not detected after once application of LPS, pan T cells increased 3 hours after second application of LPS in the connective tissue under the junctional epithelium and palatal gingival epithelium. As ED1 positive cells and Ia positive dendritic cells markedly increased after second LPS administration, It was suggested that much of these macrophages changed to Ia positive cells and increase of Ia positive antigen presenting cells might have induced appearance of T cell. In conclusion, it was demonstrated under the in vivo experimental condition that ED1 positive macrophages induced in the inflammatory lesion after LPS-stimulation might cause destruction of alveolar bone due to production of IL-1 β, and also might protect the lesion by establishment of immune response. Less

在这项研究中，我们试图通过实验阐明巨噬细胞/单核细胞系统对脂多糖（LPS）引起的边缘牙周组织炎症变化的作用。从男性 Wistar 的磨牙牙龈沟中注射一次和两次来自大肠杆菌的 LPS。大鼠牙周组织中巨噬细胞的动态、IL-1β等细胞因子的合成以及破骨细胞和淋巴细胞的动态使用免疫组织化学方法进行了研究，由于该研究是继之前的研究项目之后进行的，因此，使用新获得的数据再次报告了部分结果。 ED1阳性巨噬细胞和Ia阳性巨噬细胞尤其在牙龈上皮下结缔组织中增加，表明其中一些巨噬细胞可能产生IL-1β并激活破骨细胞牙槽骨吸收。 2.两次应用LPS导致早期ED1阳性细胞和Ia阳性细胞显着增加，怀疑这些Ia阳性细胞可能参与病灶内特异性免疫反应的建立。一次应用LPS后未检测到B细胞，第二次应用LPS后3小时，交界上皮下结缔组织和腭龈上皮中泛T细胞增多，如ED1阳性细胞和Ia。第二次LPS给药后，阳性树突状细胞显着增加，这表明这些巨噬细胞大部分转变为Ia阳性细胞，Ia阳性抗原呈递细胞的增加可能诱导了T细胞的出现。 LPS刺激后炎症病变中诱导的ED1阳性巨噬细胞可能会因产生IL-1β而导致牙槽骨破坏，也可能通过建立免疫反应来保护病变。