Analysis of trans-placental HIV infection

经胎盘HIV感染分析

基本信息

批准号：
10671572
负责人：
HAYAKAWA Satoshi
金额：
$ 0.9万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671572/
关键词：
HIV chemokine vertical transmission IL-16 経胎盤感染ケモカインレセプター Th1 Th2 CD4

项目摘要

Recent advances in perinatal care for HIV infected mothers decreased vertical transmission rates as low as 2% in Japan. However the mechanism of transplacental infection of HIV is still enigmatic and uncontrollable. In this study, we described placental pathology in HIV infected mothers in order to examine patho-physiology of congenital HIV infection and morphological basis of possible placental barriers.Subjects and methods Twelve placental samples were obtained from HIV infected mothers by AIDS working group of Japan. All cases were delivered by elective caesarean section before onset of labour. Eleven of 12 patients received oral AZT administration. No neonates delivered showed evidence of congenital infection. We examined placentae by HE stain, TUNEL and immunohistochemiistry. Various components of placental cells were separated by MACS coated with anti EGF-R, erb-B2 and CD11b which recognize villous trophoblasts (VT), extravillos trophoblasts (EVT) and Hofbauer cells (Hob) respectively. Presence of HIVgp120 RNA was examined by RT-PCR.Results By routine histological study, we observed increased frequency of intervillitis and apoptosis in placentae delivered from HIV infected patients. By quantitative analysis using TUNEL stain, we observed increased numbers of apoptotic scores. Immunohistochemical stain revealed presence of HIV p24 antigen in 2 of 12 placentae. P24 was localized both in VT and Hob. Using antigenically separated placental cells, we detected HIV gp120 RNA both in VT, EVT and Hob.Conclusion Previous reports suggest possible placental barrier to explain low transmission rates of HIV from infected mothers However we suggest, possible postplacental barriers which protect vertical transmission of HIV from infected placentae to fetuses.

日本艾滋病毒感染母亲围产期护理方面的最新进展将垂直传播率降低至 2%。然而，HIV经胎盘感染的机制仍然是个谜且无法控制。在这项研究中，我们描述了HIV感染母亲的胎盘病理学，以检查先天性HIV感染的病理生理学和可能的胎盘障碍的形态学基础。对象和方法日本艾滋病工作组从HIV感染母亲中采集了12个胎盘样本。所有病例均在临产前通过选择性剖腹产术分娩。 12 名患者中有 11 名接受口服 AZT 治疗。分娩的新生儿均未显示出先天性感染的证据。我们通过HE染色、TUNEL和免疫组织化学检查胎盘。通过涂有抗EGF-R、erb-B2和CD11b的MACS分离胎盘细胞的各种成分，这些抗EGF-R、erb-B2和CD11b分别识别绒毛滋养层(VT)、绒毛外滋养层(EVT)和霍夫鲍尔细胞(Hob)。通过RT-PCR检测HIVgp120 RNA的存在。结果通过常规组织学研究，我们观察到HIV感染者产下的胎盘中绒毛间炎和细胞凋亡的频率增加。通过使用 TUNEL 染色进行定量分析，我们观察到细胞凋亡分数增加。免疫组织化学染色显示 12 个胎盘中有 2 个存在 HIV p24 抗原。 P24 定位于 VT 和 Hob。使用抗原分离的胎盘细胞，我们在 VT、EVT 和 Hob 中检测到了 HIV gp120 RNA。结论先前的报告表明可能存在胎盘屏障来解释受感染母亲的 HIV 传播率较低。但是我们认为，可能存在胎盘后屏障，可以保护 HIV 的垂直传播胎盘传染给胎儿。