Studies on the genetical and immunological background of the genesis of preeclampsia by analyzing HLA-class II genotype.

通过分析HLA-II类基因型研究子痫前期发生的遗传和免疫学背景。

• 批准号: 10671524
• 负责人: TAKAKUWA Koichi
• 金额: $ 1.73万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671524/
• 关键词: preelampsia; HLA; infertility; antiphospholipid antibodies; genetics; immunology; HLA; DNAタイピング

Preeclampsia is a relatively common, yet mysterious, disease in pregnancy, of which the etiology has not yet been fully elucidated. Recently, genetical and immunological factors have been considered to be implicated in the genesis of preeclampsia. The major histocompatibility complex antigen complex of human beings, comprising the human leukocyte antigens (HLA), is useful in examining the immunogenetic basis of some diseases, including preeclampsia. Thus, we analyzed the antigen systems of HLA in patients with preeclampsia using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. In addition to this analysis, we also investigated the association of HLA antigen system with infertility, and the implication of autoantibodies, such as antiphospholipid antibodies, in the genesis of both preeclampsia and infertility. The results obtained in this research project are as follows ;1. The frequency of HLA-DRB1*04 and -DRB1*0403 in patients with preeclampsia, pos … More itive for anticardiolipin antibody, was significantly higher than that in normal fertile women, although the distribution of each HLA-DRB1 genotype was not significantly different between the patients with preeclampsia negative for anticardiolipin antibody and normal fertile women.2. The ferquency of HLA-DRB1*0403 and -DRB1*0410 was significantly higher in patients with infertility positive for anticardiolipin antibody compared with that in normal fertile women, and that of HLA-DQB1*0501 was significantly lower compared with that in normal fertile women.3. The frequency of HLA-DPB1*04 and -DPB1*0402 in patients with unexplained recurrent abortion was significantly higher compared with that in the general population.4. The sera positive for anticardiolipin antibody were observed to show the suppressive effect on the ィイD13ィエD1H-thymidine uptake of human umbilical endothelial cells in cell culture systems, which suggests possible mechanisms of the genesis of preeclampsia and infertility by the antiphospholipid antibodies. Less

Preclamsia在怀孕中是一种相对常见但神秘的疾病，病因尚未完全阐明。最近，遗传和免疫因子被认为是在Precmam的起源中被浸渍。人类的主要组织相容性复合物抗原复合物完成了人类白细胞抗原（HLA），可用于检查包括Precmam在内的某些疾病的免疫原性。使用聚合酶链反应限制片段长度多态性（PCR-RFLP）方法分析了HLA的抗原系统。除了进行分析外，我们还研究了HLA抗原系统与不育症的关联，以及自身抗体的含义，例如抗磷脂抗体，在前剂和不孕症的起源中。该研究项目中获得的结果如下; 1。 hla-drb1*04和-drb1*0403的频率在pos中的患者，对抗抑制蛋白抗体的意识更高，远高于正常妇女的抗生素抗体，尽管每种HLA-DRB1基因型的分布并未显着差异。与正常肥沃的妇女相比，抗多脂蛋白抗体阳性阳性的患者HLA-DRB1*0403和-DRB1*0410的原样明显更高，而HLA-DQB1*0501的抗体则明显更低。与普通人群相比，意外复发性流产的患者中HLA -DPB1*04和-DPB1*0402的频率显着高。4。观察到抗多脂蛋白抗体的血清阳性在细胞培养系统中显示了人类脐带内皮细胞的2013D1H-胸腺胺摄取的抑制作用，这表明了抗磷脂抗体的eCeclamsia和不育症的发生的可能机制。较少的

项目成果

I. Hataya, et al.: "Human leukocyte antigen class II genotype in patients with recurrent fetal miscarriage who are positive for anticardiolipin antibody."Fertil. Steril.. 70. 919-923 (1998)

I. Hataya 等人：“抗心磷脂抗体呈阳性的反复胎儿流产患者的人类白细胞抗原 II 类基因型。”Fertil。

I. Hataya: "Human leukocyte antigen class II genotype in patients with recurrent fetal miscarriage who are positive for anticardiolipin antibody"Fertility and Sterility. 70. 919-923 (1998)

I. Hataya：“抗心磷脂抗体阳性的反复胎儿流产患者的人类白细胞抗原 II 类基因型”生育力和不育。

K. Takakuwa: "Studies on the HLA-DRB1 genotypes in Japanese women with severe pre-eclampsia positive and negative for anticardiolipin antibody using a polymerase chain reaction-restriction fragment length polymorphism"Human Reproduction. 14. 2980-2986 (19

K. Takakuwa：“使用聚合酶链反应-限制性片段长度多态性对患有严重先兆子痫的日本女性抗心磷脂抗体呈阳性和阴性的 HLA-DRB1 基因型进行研究”人类生殖。

K. Takakuwa: "Possible susceptibility of the HLA-DPB1^*0402 and HLA-DPB1^*04 alleles to unexplained recurrent abortion. Analysis by means of polymerase chain reaction-restricted fragment length polymorphism method"American Journal of Reproductive Immunolo

K. Takakuwa：“HLA-DPB1^*0402 和 HLA-DPB1^*04 等位基因对不明原因复发性流产的可能易感性。通过聚合酶链反应限制片段长度多态性方法进行分析”美国生殖免疫学杂志

K. Takakuwa: "Studies on the HLA-DRB1 genotypes in Japanese women with severe pre-eclampsia positive and negative for anticardiolipin antibody using a polymerase chain reaction-restriction fragment length polymorphism method"Human Reproduction. 14. 2980-2

K. Takakuwa：“使用聚合酶链反应-限制性片段长度多态性方法研究抗心磷脂抗体阳性和阴性的日本重度子痫前期女性的 HLA-DRB1 基因型”人类生殖。