Role of Chemokines in Acute Rejection after Rat Lung Transplantation. Interaction with Nitric Oxide and Endothelin.

趋化因子在大鼠肺移植后急性排斥反应中的作用。

• 批准号: 10671257
• 负责人: MINAMI Masato
• 金额: $ 1.98万
• 依托单位: Osaka University Graduate School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671257/
• 关键词: Rat lung transplantation; Nitric Oxide; iNOS; Acute Rejection; BAL cell; 急性拒絶反応; ラット; 肺移植; 拒絶反応; ケモカイン; NO; エンドセリン

Rat lung transplantation was performed in isografts and allografts. In isografts, no acute rejection and no significant increase in NO production was identified. In allografts, grade I-II of acute rejection was seen on postoperative day (POD) 3 and grade III on POD 5. NO produced by BAL cells increased on both POD 3 (11.8±2.0 parts per billion [ppb] and POD 5 (115.3±66.9 ppb). There was a highly significant correlation between the level of NO and the severity of AR (p<0.005). BAL cells from allografts expressed iNOS mRNA.Among them, macrophages, lymphocytes and neutrophils were immunostained for iNOS.In conclusion, NO production by BAL cells was detected in the early stages of rejection. Therefore it may serve as a sensitive indicator of AR in lung transplantation.

在同种移植物和同种异体移植物中进行大鼠肺移植，在同种移植物中，没有发现急性排斥反应，并且在同种异体移植物中，在术后第 3 天（POD）出现了 I-II 级急性排斥反应，在 POD 上出现了 III 级排斥反应。 5. BAL 细胞产生的 NO 在 POD 3（11.8±2.0 ppb）和 POD 5 上均增加(115.3±66.9 ppb) NO水平与AR严重程度之间存在高度显着相关性(p<0.005)，其中巨噬细胞、淋巴细胞和中性粒细胞进行iNOS免疫染色。总之，BAL 细胞产生的 NO 在排斥反应的早期就被检测到，因此它可以作为肺移植中 AR 的敏感指标。

项目成果

T Utsumi: "Nitric oxide production by bronchoalveolar cells during allograft rejection in the rat"Transplantation. 67・12. 1622-1626 (1999)

T Utsumi：“大鼠同种异体移植排斥过程中支气管肺泡细胞产生的一氧化氮”67・12 1622-1626（1999）。

T Utsumi. M Minami.: "Nitric oxide production by bronchoalveolar cells during allograft refection in the rat"Transplantation. 67. 1622-1626 (1999)

内海 T.