Influence of estrogen on reproductive organization in perinatal mouse brain, with special reference to xenoestrogen

雌激素对围产期小鼠大脑生殖组织的影响，特别是异雌激素

基本信息

批准号：
10670032
负责人：
SEIKI Kanji
金额：
$ 0.77万
依托单位：
Tokai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

We have set out to obtain an information of whether high E levels during pregnancy would affect the development of perinatal mouse brain, especially the sex-related area through an estrogen receptor (ER) mechanism. We also examined the influence of habitual smoking during pregnancy on perinatal brain development by ad libitum administration of a nicotine-water solution.We have investigated the effects of administration of estriol (EィイD23ィエD2) and/or nicotine to pregnant mouse on neonatal offspring brain development, using immunohistochemical and radiobiochemical techniques.Estriol caused significant decreases in brain weight and ER concentration after birth, whereas nicotine plus EィイD23ィエD2 brought about no changes in brain weight or ER concentration. Intensities of ER- and neuron-specific enolase (NSE)-immunostainings were both enhanced by EィイD23ィエD2 in the reproductive organization including the preoptic area (POA), anterior to middle medial basal hypothalamus (AMBH) and amygdala (AM) after birth, Nicotine alone did not enhance the ER-immunostain intensity in POA, AMBH or AM, whereas it did enhance the NSE-immunostain intensity in these brain areas. On the contrary, simultaneous administration of nicotine and EィイD23ィエD2 brought about the reverse effect of ER- and NSE-immunostain intensities.These results indicate that estrogen treatment during the perinatal period influences, through its receptor, the development and growth of the reproductive organization in the fetal brain. The influence of nicotine needs to be further elucidated.

我们着手了解怀孕期间高E水平是否会通过雌激素受体（ER）机制影响围产期小鼠大脑的发育，特别是与性别相关的区域。我们还研究了怀孕期间习惯性吸烟对小鼠大脑发育的影响。通过随意施用尼古丁水溶液来促进围产期大脑发育。我们研究了给怀孕小鼠施用雌三醇 (EiiD23ieD2) 和/或尼古丁对新生后代大脑发育的影响，使用雌三醇导致出生后脑重量和 ER 浓度显着下降，而尼古丁加后不会导致脑重量或 ER 浓度发生变化。 ER 和神经元特异性烯醇化酶 (NSE) 免疫染色的强度均增强。 EiD23 在生殖组织中，包括视前区 (POA)、前内侧基底层出生后，单独使用尼古丁并不能增强下丘脑（AMBH）和杏仁核（AM）中POA、AMBH或AM的ER免疫染色强度，而同时施用尼古丁却可以增强这些脑区的NSE免疫染色强度。尼古丁和 E-D23 引起 ER- 和 NSE- 免疫染色强度的相反作用。这些结果表明，围产期雌激素治疗通过影响，其受体，对胎儿大脑中生殖组织的发育和生长的影响有待进一步阐明。