Inheritance of morphological and physiological characters in the inter-subspecific cross between Samia cynthia ricini and S. c. pryeri.

蓖麻和蓖麻亚种间杂交形态和生理性状的遗传。

基本信息

批准号：
10660054
负责人：
SHIMADA Toru
金额：
$ 2.24万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660054/
关键词：
Samia cynthia ricini Samia cynthia pryeri Diapause Cold torelance Sex determination Trehalose Supercooling point Chromosome 蛹休眠斑紋繭色伴性遺伝乙染色体妊性

项目摘要

The F1 hybrids were obtained from a cross between Samia cynthia pryeri ♀× Samia cynthia ricini ♂. The F1 female pupae did not enter diapause, whereas most of the F1 male pupae enter diapause, suggesting that the pupal diapause nature is inherited in a sex linked manner. In the backcross progeny, the pupal diapause seemed to be sex linked. In F1 and B1 generations, the pupae did not show diapause under a long day photoperiod. The diapausing pupae of S. c. pryeri and the F2 hybrids were incubated at 4℃ vs. 25℃ for 100 days, and their trahalose content and supercooling point in the hemolymph were measured comparatively. In 25℃- and 4℃-incubated pupae of S. c. pryeri, the trehalose content was 15mg/ml and 34mg/ml, respectively. In 25℃- and 4℃-incubated pupae in the F2 generation, it was 14mg/ml and 18mg/ml (average), respectively. In 4℃-incubated pupae in the F2, trehalose content varied from 5 to 37 mg/ml among individuals. The supercooling point was -27℃ in the 25℃-incubated pupae and -21℃ at 4℃-incubated ones, in average. No correlation was observed between the supercooling point and trahalose content in F2 individuals.Based on my observation of the backcross progenies, it was clarified that the green skin color in S. c. pryeri was controlled by a dominant gene on an autosome. The black spots on the skin of S. c. pryeri was also controlled by a dominant gene on another autosome. The cocoon of S. c, pryeri was brown. The cocoon color was segregated in the B1 generation, and various kind of colors were observed. Some genetical analyses led me to explain the cocoon color by a model ; A gene(s) and another gene(s) control a red pigment and a gray pigment, respectively.

F1 杂种是由 Samia cynthia pryeri ♀× Samia cynthia ricini ♂ 杂交获得的。F1 雌性蛹没有进入滞育，而大多数 F1 雄性蛹进入滞育，表明蛹滞育性质是性别连锁遗传的。在回交后代中，F1 和 B1 中的蛹滞育似乎是性别相关的。长日光周期下，滞育蛹和F2杂种在4℃和25℃培养100天，观察其血淋巴中的海藻糖含量和过冷点。比较了25℃和4℃孵化的S. c. 蛹中的海藻糖含量。在25℃和4℃孵化的F2代中分别为15mg/ml和34mg/ml，在4℃孵化的F2代中分别为14mg/ml和18mg/ml。，海藻糖含量在个体之间变化为5至37mg/ml。25℃培养中的过冷点为-27℃。蛹和-21℃在4℃孵化的蛹中，F2个体的过冷点和海藻糖含量之间没有观察到任何相关性。根据我对回交后代的观察，澄清了蛹的绿色皮肤颜色。 c. pryeri 受常染色体上的显性基因控制。 S. pryeri 皮肤上的黑斑也受另一个常染色体上的显性基因控制。 c，Pryeri 的茧颜色在 B1 代中被分离，并且观察到了各种颜色，使我通过模型解释了茧的颜色；分别为红色颜料和灰色颜料。