Study on the conservation of genetic diversity in Calanthe triplicata

三瓣虾兰遗传多样性保护研究

基本信息

批准号：
10660039
负责人：
MIZUTANI Takayuki
金额：
$ 1.86万
依托单位：
KYUSHU TOKAI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

Ten of thirteen isozymes used for the analysis in Calanthe triplicata, DIA, FDH, GDH, GOT, MDH, ME, 6-PDH, PGI, PGM, and SADH, showed the stable zymogram patterns. Of these, seven enzymes, GOT, IDH, MDH, ME, PGI, 6-PDH and SADH, showed intraspecific polymorphisms in C.triplicata. Especially, GOT, MDH and 6-PDH were found showing highly comparing with other terrestrial orchids grow wild in Iriomote Island might contribute the wide distribution of this species. All individual plants of Calanthe triplicata included in three populations were analyzed by seven isozymes systems. In all the three populations, the big diversities of the isozymes were observed. Most of plants investigated showed the variation and the high heterozygousity in the population of C.triplicata. Seventeen of eighteen individuals in the population B can be identified using only four or five isozyme systems. The isozyme variation observed in the population B showed that the leading propagation strategy of the C.triplicata is seedling. Only two plants could not be distinguished from each other by the seven isozyme systems. They located very closely in the population, suggesting that the two plants elonged to the same colony or one of them was an offspring or a pod mate of the other. The considered to be maintained by a seed propagation, especially outbreeding. The species was found to have a big genetic diversity even in a wild population, suggesting that Calanthe triplicata is an allogamous species propagating itself by seedling.

用于分析三棱脊兰的 13 种同工酶中，有 10 种显示出稳定的酶谱模式：DIA、FDH、GDH、GOT、MDH、ME、6-PDH、PGI、PGM 和 SADH。其中，GOT、IDH、MDH、ME、PGI、6-PDH 和 SADH 七种酶在 C.triplicata 中表现出种内多态性。特别是，与西表岛野生的其他陆生兰花相比，GOT、MDH 和 6-PDH 的含量较高，这可能有助于该物种的广泛分布。通过七个同工酶系统分析了三个种群中的所有三叶脊兰单株植物。在所有三个群体中，观察到同工酶的巨大多样性。所调查的大多数植物在C.triplicata群体中表现出变异和高杂合性。仅使用四到五个同工酶系统就可以识别群体 B 中 18 个个体中的 17 个。在种群 B 中观察到的同工酶变异表明，C.triplicata 的主要繁殖策略是育苗。只有两种植物无法通过七个同工酶系统来区分。它们在群体中的位置非常接近，表明这两种植物延伸到同一群体，或者其中一种是另一种的后代或荚伙伴。被认为是通过种子繁殖，尤其是远交来维持的。研究发现，即使在野生种群中，该物种也具有很大的遗传多样性，这表明 Calanthe Triplicata 是一种通过幼苗自行繁殖的异体受精物种。