Study on Isolation and Biochemical Examination of Psychrotrophic Bacterium for Large Scale Production of Antimicrobial Violet Pigment

大规模生产抗菌紫色颜料的耐冷菌分离及生化检测研究

基本信息

批准号：
10650780
负责人：
NAKAMURA Yoshitoshi
金额：
$ 2.18万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10650780/
关键词：
violet pigment psychrotrophic bacterium antimicrobial activity putrefactive bacterium natural pigment

项目摘要

About 20 % of all foodstuffs produced in the world are wasted because of septic action by microorganisms and many people have experienced bad health effects due to the ingestion of septic food. Since many microorganisms have been reported as putrefactive and pathogenic bacteria, which cause the septic action of food, the prevention of bacteria contamination in food storage is one of the most important factors for supplying safe and healthy food to the consumer. The authors have screened a psychrotrophic bacterium, RT102 strain, which synthesized an antimicrobial violet pigment, from the intestine of a rainbow trout and found out that the violet pigment shows an antimicrobial action for some species of microorganisms. The chemical structure and antimicrobial activity of violet pigment produced by the psychrotrophic bacterium isolated from the intestine of a rainbow trout were investigated experimentally. The psychrotrophic bacterium was found to be a new species belonging to the genus Janthinobacterium lividum. ィイD11ィエD1H, ィイD113ィエD1C-NMR, and FT-MS spectra analyses results showed that the chemical structure of violet pigment was a mixture of vioracein and deoxyvioracein. The antimicrobial activity of violet pigment was confirmed for putrefactive bacteria such as Bacillus subtilis, Bacillus megaterium, Staphylococcus aureus, and Pseudomonas aeruginosa. The growth inhibitory and lethal effects of violet pigment on the putrefactive bacteria were evaluated by increasing the concentration of violet pigment, ranging from 5 to 20 mg/1. It was found that higher concentrations of violet pigment caused not only growth inhibition but also the death of the putrefactive bacteria.

由于微生物的化粪池作用，世界上生产的所有食品中约有20％被浪费了，许多人由于摄入化粪池而造成了不良的健康影响。由于据报道许多微生物是腐败性和致病性细菌，这些细菌引起食物的化粪池作用，因此预防细菌在食品储存中的污染是为消费者提供安全健康食品的最重要因素之一。作者已经从彩虹鳟鱼的肠道中筛选了一种基质细菌RT102菌株RT102菌株，该菌株合成了一种抗菌紫色的色素，发现紫罗兰色的色素显示出一些微生物的抗菌作用。从彩虹鳟鱼的肠道中分离出的精神营养细菌产生的紫罗兰色色素的化学结构和抗菌活性进行了实验研究。人们发现，基属营养细菌是新的抑制性生长抑制作用，紫罗兰色色素的生长抑制活性被证实，例如腐烂细菌，例如枯草芽孢杆菌，巨型巨芽孢杆菌，金黄色葡萄球菌，金黄色葡萄球菌和铜绿硫酸葡萄球菌。通过增加紫罗兰色色素的浓度，评估了紫罗兰色色素对腐败细菌的致命作用，范围从5至20 mg/1。发现较高浓度的紫色色素不仅引起生长抑制作用，而且会导致腐烂细菌的死亡。