Measurement of Concentration Distributions of Protein Layer Deposited on Membrane in Inclined Ultrafiltration of Protein Solutions

蛋白质溶液倾斜超滤中沉积在膜上的蛋白质层浓度分布的测量

基本信息

批准号：
10650758
负责人：
IRITANI Eiji
金额：
$ 2.24万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10650758/
关键词：
Ultrafiltration Protein Concentration Distribution Inclined Filtration BSA Deposited Layer Fouling Filter Cake 下向流濾過

项目摘要

A dramatic flux decline during ultrafiltration is the thorniest problem in the use of ultrafiltration systems. Such flux decline is mainly attributed to the accumulation of the protein molecules on the membrane surface. Therefore, an understanding of the properties of the protein layer accumulated on the membrane surface can serve as a basis for clarifying the real mechanism of ultrafiltration. In this study, a method has been developed for measuring the concentration variations of the accumulated protein layer tends to have a much more compact structure at the membrane in comparison to a relatively loose condition at the surface. This is the same tendency as the filter cake formed in filtration of particulate suspensions. It was also shown that the thickness of the protein layer increases as the filtration proceeds. This method is of significance for clarifying the real mechanism of ultrafiltration, become few reliable experimental data on the concentration distributions of the protein layer have been reported so far.The compressible cake filtration model has been widely used to describe filtration behaves of particulate suspensions. Recently, the model has been used to explain the mechanism of ultrafiltration. In this study, the experimental results of the concentration variations within the protein layer were compared with the calculations on the basis of the compressible cake filtration model. The measured concentration variations accorded well with the calculated results, which revealed that the dynamic deposition behaviors of the protein layer in ultrafiltration can be accurately described by the compressible cake filtration model.

超滤期间的急剧下降是使用超滤系统中最棘手的问题。这种通量下降主要归因于蛋白质分子在膜表面的积累。因此，了解积累在膜表面上的蛋白质层的性质可以作为阐明超滤的真实机制的基础。在这项研究中，已经开发了一种方法来测量累积蛋白质层的浓度变化，而与表面相对较宽的状况相比，膜上的结构更紧凑。这与在颗粒悬浮液过滤时形成的过滤蛋糕相同。还表明，随着过滤的进行，蛋白质层的厚度增加。该方法对于阐明超滤的真实机制具有重要意义，到目前为止，已经报道了有关蛋白质层浓度分布的可靠实验数据。可压缩的蛋糕过滤模型已广泛用来描述颗粒悬浮液的过滤行为。最近，该模型已用于解释超滤机制。在这项研究中，将蛋白质层内浓度变化的实验结果与根据可压缩蛋糕过滤模型的计算进行了比较。测量的浓度变化与计算结果很好，这表明超滤中蛋白质层的动态沉积行为可以通过可压缩的蛋糕过滤模型准确地描述。