Reaction between deep brain photoreceptors and circadian clock mechanism in vertebrates

脊椎动物深部脑光感受器与生物钟机制之间的反应

• 批准号: 10640666
• 负责人: OISHI Tadashi
• 金额: $ 2.5万
• 依托单位: Nara Women's University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10640666/
• 关键词: vertebrates; deep brain; photoreceptor; circadian clock; rhodopsin; retinal; serotonin; VIP

In 1998, we obtained following results : (1)cell groups with strong immunoreactivity to anti- serotonin antibody were found in the para-ventricular nucleus of the hypothalamus in Japanese quail. The number of immunopositive cells was greater in light than in darkness. Confocal razar microscopy revealed that these cells extend processes perpendicular to the third ventricle and connect each other. (2)Vasoactive intestinal peptide (VIP) immunopositive cells were found at the ventral area of the para-ventricular nucleus. These cells extend fibers parallel to the third ventricle and were suggested to connect with serotonin immunopositive cells.In 1999, following results were obtained : (1)The antibody against the toad rhodopsin stained the suprachiasmatic nucleus and preoptic nucleus. (2)This antibody also stained the exocrine glands in the head skin. (3)Fluorescence method with formaldehyde revealed a possibility of existence of retinal in these areas. (4)Action spectrum experiments with using a large spectrograph revealed that pinopsin is the possible photo-pigment involved in photo-suppression of melatonin production in the pineal

1998年，我们得到如下结果：(1)在日本鹌鹑下丘脑室旁核中发现了抗血清素抗体具有强免疫反应性的细胞群。光照下免疫阳性细胞的数量多于黑暗下的。共聚焦显微镜显示这些细胞垂直于第三脑室延伸突起并相互连接。 (2)室旁核腹侧区发现血管活性肠肽(VIP)免疫阳性细胞。这些细胞平行于第三脑室延伸纤维，被认为与血清素免疫阳性细胞连接。1999年，获得以下结果：(1)抗蟾蜍视紫红质抗体对视交叉上核和视前核染色。 (2)该抗体还对头部皮肤的外分泌腺进行染色。 (3)甲醛荧光法揭示这些区域可能存在视网膜。 (4)使用大型光谱仪进行的作用光谱实验表明，pinopsin可能是参与光抑制松果体褪黑激素产生的光色素。

项目成果

Yamao, M., Araki, M., 他3名: "Differentiation of pinopsin-immunoreactive cells in the developing quail pineal organ : an in-vivo and in-vitro immunohistochemical study"Cell Tissue Res.. 296. 667-671 (1999)

Yamao, M.、Araki, M. 和其他 3 人：“鹌鹑松果体器官发育中的松菌素免疫反应细胞的分化：体内和体外免疫组织化学研究”细胞组织研究.. 296. 667-671 ( 1999）

大石正(編): "光と人間"朝倉書店. 184 (1999)

大石正（主编）：《光与人性》朝仓书店 184（1999）。

Yashiro,Y.,Oishi,T. 他4名: "Recent Advances in Physiological Anthropology" 研究社(in press), (1999)

Yashiro, Y.、Oishi, T. 和其他 4 人：“生理人类学的最新进展”Kenkyusha（正在出版），（1999 年）

Yamao,M.,Araki,M.,他3名: "Differentiation of pinopsin-immunor eactive cells in the developing quail pineal or gan: an in-vivo and in-vitro immunohistochemical study"Cell Tissue Res.. 296. 667-671 (1999)

Yamao, M.、Araki, M. 和其他 3 人：“正在发育的鹌鹑松果体器官中松果体蛋白免疫活性细胞的分化：体内和体外免疫组织化学研究”细胞组织研究.. 296. 667- 671 (1999)

大石正: "生物の光環境センサー(シリーズ・光が拓く生命科学、第1巻)"共立出版. 165 (1999)

大石正：“生物的光环境传感器（系列：光开创的生命科学，第 1 卷）”Kyoritsu Shuppan 165（1999）。