Transcriptional regulation of rhodopsin gene under light dark cycle

光暗循环下视紫红质基因的转录调控

• 批准号: 10470360
• 负责人: MATSUDA Hidehiko
• 金额: $ 7.87万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10470360/
• 关键词: c-fos; rhodopsin; transcription; light; promotor; 明暗環境; mGluR6; CREB; FOS蛋白; 転写制御; 明暗変化; 日内変動

The signal pathway transcriptional regulation of rhodopsin gene under light dark cycle was investigated. The oligo nucleotide of the promoter cite of the rhodopsin ran slower in the gel retardation assay when incubated with the nuclear extract of the retina, suggesting that there are something in the nucleus of the retina that bind the site.In addition, the oligo nucleotide ran even slower when incubated with anti-c-Fos antibody and the nuclear extract of the retina, suggesting that c-Fos protein in the retina bind the promoter cite of the rhodopsin gene. We are now eramining the role of the c-fos gene in the transcriptional regulation of the rhodopsin in vivo by using c-fos knockout mice.

研究了光暗周期下视紫红质基因信号通路的转录调控。当与视网膜核提取物一起孵育时，视紫红质启动子位点的寡核苷酸在凝胶延迟测定中运行较慢，表明视网膜细胞核中存在与该位点结合的东西。此外，寡核苷酸运行当与抗c-Fos抗体和视网膜核提取物一起孵育时，速度甚至更慢，这表明视网膜中的c-Fos蛋白结合视紫红质基因的启动子引用。我们现在正在通过使用 c-fos 基因敲除小鼠来消除 c-fos 基因在体内视紫红质转录调节中的作用。

项目成果

Kotake S.et al.: "Central nervous system symptoms in patients with Behcet disease receiving cyclosprine therapy"Opthalmology. 106. 586-589 (1999)

Kotake S.等人：“接受环孢素治疗的白塞病患者的中枢神经系统症状”眼科。

Tokita, R et al.: "Prolaction secretion in response to prolactin-releasing peptide and the expressin of the prolactin-releasing paptide gene in the medulla oblongata are eatrogen dependent in rats."Neurosei. Lett.. 276. 103-106 (1999)

Tokita, R等人：“在大鼠中，催乳素释放肽的催乳分泌和延髓中催乳素释放肽基因的表达是雌激素依赖性的。”Neurosei。

Kitaichi N.et al.: "Prominent increase of macrophage migration inhibitory factor in the sera of patients with uveitis"Invest Ophthamol Vis Sci. 40. 247-250 (1999)

Kitaichi N.等人：“葡萄膜炎患者血清中巨噬细胞迁移抑制因子显着增加”Invest Ophthamol Vis Sci。

Onodera H.et al.: "Differential expression of c-fos mRNA in rat neocortex by instuhybridization"Life Sci.. 64. 1127-1135 (1999)

Onodera H.等人：“通过instuhybridization在大鼠新皮质中差异表达c-fos mRNA”Life Sci.. 64. 1127-1135 (1999)

Yamada, K., et al.: "Hyper responsiveness to palatable and aversive taste stimuli in genetically obese (bonbesin recepter subtype-3-dificient) mice"Physiol Behav.. 66. 863-867 (1999)

Yamada, K. 等人：“遗传性肥胖（bonbesin 受体亚型 3 缺陷）小鼠对可口和厌恶味觉刺激的过度反应”Physiol Behav.. 66. 863-867 (1999)