Physiologic and pathologic functions of a CXC chemokine PBSF/SDF-1 and its receptor CXCR4

CXC趋化因子PBSF/SDF-1及其受体CXCR4的生理和病理功能

• 批准号: 10470092
• 负责人: NAGASAWA Takashi
• 金额: $ 3.78万
• 依托单位: Research Institute, Osaka Medical Center for Maternal and Child Health
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10470092/
• 关键词: chemokine; bone marrow; B lymphocyte; hematopoiesis; homing; angiogenesis; B細胞; 腸管

Chemokines are a family of small structurally related molecules that were recognized originally for their ability to regulate cell trafficking in inflammation. We isolated a chemokine, stromal cell-derived factor/pre-B-cell growth stimulating factor (SDF-1/PBSF) as a molecule that stimulates the growth of B lymphocyte precursors and have found its multiple physiological functions in development. We have shown that SDF-1/PBSF is essential for embryonic viability, development of B lymphocyte, colonization of bone marrow by hematopoietic cells and cardiogenesis. Moreover, we identified a murine seven-transmembrane G-protein-coupled receptor for SDF-1/PBSF, termed CXCR4, that also functions as a coreceptor for strains of HIV-1. Here we generated CXCR4 deficient mice and found that CXCR4 was a primary physiologic receptor for SDF-1/PBSF.

趋化因子是一个与结构相关的小分子的家族，最初以调节炎症中细胞运输的能力而被识别。我们将趋化因子，基质细胞衍生的因子/前B细胞生长刺激因子（SDF-1/PBSF）分离为一种分子，可刺激B淋巴细胞前体的生长，并发现其在发育中的多重生理功能。我们已经表明，SDF-1/PBSF对于胚胎生存能力，B淋巴细胞的发展，造血细胞对骨髓的定殖和心脏发生至关重要。此外，我们确定了一种称为CXCR4的鼠七跨膜G蛋白偶联受体，该受体也可作为HIV-1菌株的共肽。在这里，我们产生了CXCR4缺乏小鼠，发现CXCR4是SDF-1/PBSF的主要生理受体。

项目成果

NAGASAWA, T., Tachibana, K., Kishimoto, T.: "A novel CXC chemokine PBSF/SDF-1 and its receptor CXCR4: their functions in development, hematopoiesis and HIV infection" Semin.Immunol.10. 179-185 (1998)

NAGASAWA, T.、Tachibana, K.、Kishimoto, T.：“一种新型 CXC 趋化因子 PBSF/SDF-1 及其受体 CXCR4：它们在发育、造血和 HIV 感染中的功能”Semin.Immunol.10。

Nagasawa, T., Tachibana, K., Kawabata, K.: "A CXC chemokine SDF-1/PBSF : A ligand for a HIV coreceptor, CXCR4"Adv. Immunol.. 71. 211-228 (1999)

Nagasawa, T.、Tachibana, K.、Kawabata, K.：“CXC 趋化因子 SDF-1/PBSF：HIV 辅助受体 CXCR4 的配体”Adv。

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Kawabata, K., Ujikawa, M., Egawa, T., Kawamoto, H., Tachibana, K., Iizawa, H., Katsura, Y., Kishimoto, T., Nagasawa, T.: "A cell-autonomous requirement for CXCR4 in long-term lymphoid and myeloid reconstitution"Proc. Natl. Acad. Sci. USA. 96. 5663-5667 (1

Kawabata, K.、Ujikawa, M.、Egawa, T.、Kawamoto, H.、Tachibana, K.、Iizawa, H.、Katsura, Y.、Kishimoto, T.、Nagasawa, T.：“细胞自主

Tachibana, K., Hirota, S., Iizawa, H., Yoshida, H., Kawabata, K., Kataoka, Y., Kitamura, Y., Matsushima, K., Yoshida, N., Nishikawa, S., Kishimoto, T., & NAGASAWA, T.: "The chomokinase receptor CXCR4 is essential for vascularization of the gastrointertina

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