Study on dynamic mechanism of perforation on cancer cells at shock waves exposures

冲击波作用下癌细胞穿孔的动力学机制研究

基本信息

批准号：
10450071
负责人：
TAKAYAMA Kazuyoshi
金额：
$ 8.06万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10450071/
关键词：
Underwater shock waves Tissue model Holographic interferometry Numerical simulation Perforation Cancer cell Shock wave 値シミュレーション

项目摘要

Extracoporeal shock wave lithotripsy (ESWL), non-invasive removal of kidney stones, is one of the most peaceful applications of shock waves. As a collaboration project with Medical School of Tohoku University we have developed prototype lithotriptor which was approved by the Ministry of Health and Welfare. As a result of basic research, tissue damages during ESWL treatments have been clarified and we found a technology to damage living tissue in a controlled fashion. This technology is extended to cancer research with the combination of chemotherapy during which it was discovered punctuation on the cancer cells when shock waves were exposed on them. The mechanism of punctuation is found to be related with shock wave interaction with nonhomogeneity of a medium with local curvature. The goal of present research is to clarify the mechanism of the punctuation in details.Results obtained are summarized as following :(1) Optical flow visualization of shock/tissue interaction has been developed. However, it has a limitiation in its spatial resolution. In order to compensate it, we seek for model tissues which have analogous characteristics to living tissues, with which various analogue experiments have been conducted and the results have been numerically simulated.(2) Finite fringe holographic interferometry has been developed and Fourier fringe analysis was well established. This method allowed to identify a slight density change created by shock wave exposures and was found to be effectively extended further shock/tissue interaction studies.(3) Numerical methods have been developed to be compared with experiments. Interactions between shock waves and various gas liquid interfaces have been successfully simulated.(4) A vertcal gas gun has been designed and constructed with which micro-shock/tissue interaction will be investigated. This is a facility which can directly simulate shock induced perforation on model tissue surface.

室外休克波碎石（ESWL）是肾结石的无创，是电击波的最宁静的应用之一。作为与Tohoku大学医学院的合作项目，我们开发了原型岩性曲折，该原型已获得卫生和福利部的批准。基础研究的结果是，已经阐明了ESWL治疗过程中的组织损伤，我们发现了一种以受控方式损害生物组织的技术。通过化学疗法的结合，该技术扩展到癌症研究，在此期间，当冲击波暴露在癌细胞上时，它在癌细胞上被发现标点符号。发现标点符号的机制与具有局部曲率的培养基的不均匀性与冲击波相互作用有关。目前的研究的目的是详细阐明标点符号的机制。获得的结果总结如下：（1）已开发了冲击/组织相互作用的光流可视化。但是，它的空间分辨率有限制。为了补偿它，我们寻求与活组织具有类似特征的模型组织，并且已经进行了各种模拟实验并在数值上模拟了结果。（2）已经开发良好。这种方法允许识别冲击波暴露产生的轻微密度变化，并被发现有效扩展了进一步的冲击/组织相互作用研究。（3）已开发出与实验相比的数值方法。冲击波与各种气体液体界面之间的相互作用已成功模拟。（4）设计和构建了Vertcal气枪，并研究了微冲击/组织相互作用。这是一个可以直接模拟模型组织表面上的冲击引起的穿孔的设施。