Studies on Electrochemical Generation, Detection and Reaction Control of Reactive Oxygen Species

活性氧的电化学产生、检测及反应控制研究

• 批准号: 10305064
• 负责人: OHSAKA Takeo
• 金额: $ 23.91万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10305064/
• 关键词: Reactive Oxygen Species; Superoxide Ion; Self-assembled Monolayer Film; Electochemistry; Hydrogen Peroxide; Biosensor; Enzyme; Oxygen; 活性酵素種; スーパーオキシドジスムターゼ; 自己組織化膜; 電極触媒; 抗酸化物質; 酸素の拡散係数; 電気化学振動現象; 酸素ラジカル; ストップドフロー分光法

In the present study, challenging studies on the electrochemical generation, detection and reaction control of reactive oxygen species were undertaken using molecularly modified electrodes and the following remarkable results were obtained :1. The electrogeneration of O_2 was realized by one-electron reduction of O_2 in aqueous media using Hg electrodes modified with surface-active molecules.2. Novel dinickel(II) and dicopper(II) complexes of bis(pentaazamacrocycle) were prepared by a template condensation reaction and their self-assembled monolayer(SAM)s were fabricated on Au electrodes. The electrocatalytic oxidation of H_2O_2 to O_2 could be clarified by in situ FTIR measurements.3. The direct electrode reactions of SOD- and cyt.c -confined SAMs could be realized and these SAM-electrodes were successfully applied to third generation biosensors for O_2^-.4. A series of asymmetric viologen monolayers fabricated on electrodes were found to show electrochemical monomer/dimmer equilibria on CVs with an isopotential point.5. New electrochemical oscillation phenomena associated with in situ electrogenerated O_2^- were found and their mechanisms were proposed.

在本研究中，使用分子修饰的电极进行了对电化学产生，反应性氧的检测和反应控制的挑战研究，并获得了以下显着的结果：1。通过使用表面活性分子修饰的HG电极在水性培养基中的O_2中，O_2的O_2一单电子降低来实现O_2的电气化。2。通过模板凝结反应制备了新型的dinickel（ii）和双子（pentaazamacrocycle）的双子（II）络合物，并在AU电极上制造了它们的自组装单层（SAM）S。 H_2O_2至O_2的电催化氧化可以通过原位FTIR测量来阐明3。可以实现SOD和CYT.C构造的SAM的直接电极反应，并将这些SAM-电极成功应用于O_2^ - .4的第三代生物传感器。发现在电极上制造的一系列不对称的Viologen单层可以显示具有等电位点的CVS上的电化学单体/调光器平衡。5。发现了与原位电源的O_2^相关的新电化学振荡现象，并提出了它们的机制。

项目成果

M.I.Awad.: "Simultaneous Electroanalysis of Peroxyacetic Acid and Hydrogen Peroxide"Anal.Chem.. 73. 1839-1843 (2001)

M.I.Awad.：“过氧乙酸和过氧化氢的同时电分析”Anal.Chem.. 73. 1839-1843 (2001)

D.Zhang: "Electrochemical Reduction of Dioxygen at Mercury Electrode in Alkaline Aqueous Media"Jpn.J.Deutrium Sci.. (in press). (2002)

D.Zhang：“碱性水介质中汞电极处的分子氧的电化学还原”Jpn.J.Deutrium Sci..（出版中）。

K. Arihara, T. Ohsaka and F. Kitamura: "Characteristic Cyclic Voltammograms of Alkyl Viologens at Single Crystal Gold Electrodes;"Phys. Chem. Chem. Phys.. (in press.).

K. Arihara、T. Ohsaka 和 F. Kitamura：“烷基紫精在单晶金电极上的特征循环伏安图”；Phys。

S. Kishioka, S. Ohki, T. Ohsaka and K. Tokuda: "Reaction Mechanism and Kinetics of Alcohol Oxidation at Nitroxyl Radical Modified Electrodes"J. Electroanal. Chem.. 452. 179-186 (1998)

S. Kishioka、S. Ohki、T. Ohsaka 和 K. Tokuda：“硝酰自由基修饰电极上酒精氧化的反应机理和动力学”J。

T.OKAJIMA: "Chemiluminescense of Indole and Its Derivatives induced by Electrogeneradted Superoxide Ion…"Electrochem. Acta. (発表予定). (2002)

T.OKAJIMA：“电化学超氧化物离子诱导的吲哚及其衍生物的化学发光……”《电化学》（待发表）。