Immunocytochemical study of trans-Golgi-network (TGN)-associated protein in salivary acinar cells under varying condition of salivary secretion

不同唾液分泌条件下唾液腺泡细胞中跨高尔基体网络（TGN）相关蛋白的免疫细胞化学研究

基本信息

批准号：
08672101
负责人：
KURABUCHI Shingo
金额：
$ 1.34万
依托单位：
The Nippon Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672101/
关键词：
Rab3 Rab4 Rab6 Trans-Golgi network (TGN)secrection granules Sorting Secretory cells Salivary gland rab3A rab3B rab6 β cop γ adaptin ゴルジ装置 r ab3A r ab3B r ab6

项目摘要

Specific antisera against Rab3A, Rab3B, Rab3D, Rab4, and Rab6, small CTP-binding proteins, and TGN38, a marker of trans-Golgi network (TGN), have been used to examine the distribution of these Rab proteins in several endocrine and exocrine glands of mouse and rat by immunofluorescence method. The anti-Rab3A and Rab6 immunostained some rat pituitary endocrine cells, It is conceivable by these distribution that the Rab3A-immunoreactive cells may be ACTH cells. The anti-Rab3A immunostained exclusively several endocrine cells of pancreatic islets of rat. When cosecutive sections were immunostained with antibodies against pancreatic hormones, these immunostained cells were found to be alpha cells. In contrast, anti-Rab3D immunostained pancreatic acinar cells, and the immunoreacted region localized to the zymogen granule field. Double electron microscopic immunogold localizations for Rab3A or Rab3D and TGN38 have been tried in these materials, but no optimal resolution has not obtained by electron microscope. However, it was found in the light microscopy that these immunoreactive localization of Rab proteins should be in the field of secretion granules. Because of the Rab associated with secretion granules, Rab proteins may play a role in regulated exocytosis.

针对 Rab3A、Rab3B、Rab3D、Rab4 和 Rab6（小 CTP 结合蛋白）和 TGN38（跨高尔基体网络 (TGN) 标记）的特异性抗血清已用于检查这些 Rab 蛋白在多种内分泌和外分泌中的分布采用免疫荧光法检测小鼠和大鼠的腺体。对一些大鼠垂体内分泌细胞进行抗Rab3A和Rab6免疫染色，根据这些分布可以想象，Rab3A免疫反应细胞可能是ACTH细胞。抗Rab3A仅对大鼠胰岛的几个内分泌细胞进行免疫染色。当连续切片用抗胰腺激素的抗体进行免疫染色时，发现这些免疫染色的细胞是α细胞。相反，抗 Rab3D 免疫染色胰腺腺泡细胞，免疫反应区域定位于酶原颗粒区域。已经在这些材料中尝试了 Rab3A 或 Rab3D 和 TGN38 的双电镜免疫金定位，但电镜尚未获得最佳分辨率。然而，在光学显微镜下发现，Rab蛋白的这些免疫反应定位应该在分泌颗粒区域。由于 Rab 与分泌颗粒相关，因此 Rab 蛋白可能在调节胞吐作用中发挥作用。