Immunocytochemical study of trans-Golgi-network (TGN)-associated protein in salivary acinar cells under varying condition of salivary secretion

不同唾液分泌条件下唾液腺泡细胞中跨高尔基体网络（TGN）相关蛋白的免疫细胞化学研究

基本信息

批准号：
08672101
负责人：
KURABUCHI Shingo
金额：
$ 1.34万
依托单位：
The Nippon Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672101/
关键词：
Rab3 Rab4 Rab6 Trans-Golgi network (TGN)secrection granules Sorting Secretory cells Salivary gland rab3A rab3B rab6 β cop γ adaptin ゴルジ装置 r ab3A r ab3B r ab6

项目摘要

Specific antisera against Rab3A, Rab3B, Rab3D, Rab4, and Rab6, small CTP-binding proteins, and TGN38, a marker of trans-Golgi network (TGN), have been used to examine the distribution of these Rab proteins in several endocrine and exocrine glands of mouse and rat by immunofluorescence method. The anti-Rab3A and Rab6 immunostained some rat pituitary endocrine cells, It is conceivable by these distribution that the Rab3A-immunoreactive cells may be ACTH cells. The anti-Rab3A immunostained exclusively several endocrine cells of pancreatic islets of rat. When cosecutive sections were immunostained with antibodies against pancreatic hormones, these immunostained cells were found to be alpha cells. In contrast, anti-Rab3D immunostained pancreatic acinar cells, and the immunoreacted region localized to the zymogen granule field. Double electron microscopic immunogold localizations for Rab3A or Rab3D and TGN38 have been tried in these materials, but no optimal resolution has not obtained by electron microscope. However, it was found in the light microscopy that these immunoreactive localization of Rab proteins should be in the field of secretion granules. Because of the Rab associated with secretion granules, Rab proteins may play a role in regulated exocytosis.

针对RAB3A，RAB3B，RAB3D，RAB4和RAB6，小CTP结合蛋白和TGN38（TGN38）的特定抗血清（TGN38）是Trans-Golgi网络（TGN）的标志物，用于检查这些Rab蛋白在几种内分泌和外分腺中通过免疫荧光方法的分布分布。抗RAB3A和RAB6免疫染色了一些大鼠垂体内分泌细胞，可以通过这些分布认为Rab3a-免疫反应性细胞可能是Acth细胞。抗RAB3A免疫染色仅是大鼠胰岛的几个内分泌细胞。当连续切片用抗胰腺激素的抗体免疫染色时，发现这些免疫染色的细胞是α细胞。相比之下，抗RAB3D免疫染色的胰腺腺泡细胞和免疫反应区域位于Zymogen颗粒场。在这些材料中已经尝试了RAB3A或RAB3D和TGN38的双电子显微镜免疫局部化，但是没有通过电子显微镜获得的最佳分辨率。然而，在光学显微镜中发现，这些Rab蛋白的这些免疫反应性定位应在分泌颗粒领域。由于与分泌颗粒相关的RAB，Rab蛋白可能在调节的胞吐作用中起作用。