Lacrimal gland transplantation for improvement of the gland function

泪腺移植以改善泪腺功能

• 批准号: 08672035
• 负责人: ONO Masafumi
• 金额: $ 1.47万
• 依托单位: Tokai University (1997-1998)Tokyo Dental College (1996)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672035/
• 关键词: lacrimal gland transplantation; dry eye; Sjogren's sydrome; lactoferrin; lactoferin; 涙液蛋白; ラクトフェリン; シェ-グレン症候群; 涙腺; 移植; 涙液

This study was originally designed to investigate die possibility of lacrimal gland transplantation as a treatment for severe dry eye, inducing Siogren's syndrome (SS). The surgical procedure of auto lacrimal gland graft transplantation has not been established and the concentration and function of transplanted lacrimal glands has not been evaluated in any animal model studies,To establish an evaluation system for lacrimal gland function, lactoferrin protein was measured in the tear through the use of LACTOPLATE TM (JDC) quantitation. Detection of lactoferrin in the lacrimal glands was accomplished by immunological staining using anti-human lactoferrin in patients with 55, (who have been documented to have less protein and secretion from the glands), and in non-SS (NSS). This antibody has been used for evaluation of the function of lacrimal glands. Previously, case studies have shown that tear lactoferrin in severe day eye including 55 is significantly lower than in NSS day eye cases. We confirmed the results of this lacrimal gland evaluation system using tie aforementioned anti-lactoferrin antibody. Furthermore, relatively high concentrations of lactoferrin protein were found to exist in the acinar and ductal cells in the 55 Iacnmal gland. This suggests that tear protein secretion disorders might occur in SS lacrimal glands instead of being caused by protein synthesis disorders. We believe this evaluation system might be of use in the examination of synthesis disorders. Additionally, this system might also be able to be used to evaluate the fine details of lacrimal gland dysfunction due to causes such as protein synthesis and/or secretion disorders in severe dry eye (including SS).

这项研究最初的目的是调查泪腺移植治疗严重干眼症（诱发西奥格伦综合征（SS））的可能性。自体泪腺移植的手术流程尚未建立，移植泪腺的浓度和功能尚未在任何动物模型研究中进行评估，为了建立泪腺功能的评估体系，通过测定泪液中的乳铁蛋白使用LACTOPLATE TM (JDC)定量。通过使用抗人乳铁蛋白对 55 岁患者（已被证明泪腺中的蛋白质和分泌物较少）和非 SS (NSS) 患者进行免疫染色来检测泪腺中的乳铁蛋白。该抗体已用于评估泪腺的功能。此前的病例研究表明，包括55在内的严重日间眼病例的泪液乳铁蛋白显着低于NSS日间眼病例。我们使用上述抗乳铁蛋白抗体确认了该泪腺评估系统的结果。此外，在 55 腺体的腺泡和导管细胞中发现了相对高浓度的乳铁蛋白。这表明泪液蛋白分泌障碍可能发生在SS泪腺中，而不是由蛋白质合成障碍引起。我们相信该评估系统可能可用于检查综合障碍。此外，该系统还可以用于评估由于严重干眼症（包括 SS）中蛋白质合成和/或分泌障碍等原因引起的泪腺功能障碍的细节。