Fundamental experiments of bacterial translocation in clinical setting (New development for diagnosis of bacterial translocation)

临床环境中细菌易位的基础实验（细菌易位诊断的新进展）

• 批准号: 08671436
• 负责人: HANASAWA Kazuyoshi
• 金额: $ 1.47万
• 依托单位: Shiga University of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671436/
• 关键词: Endotoxin; Bacterial Translocation; Endotoxin Translocation; mesentric lymph node; Polyethylene glycol; TNF; m-RNA; hemorrhagic shock; Bacterial translocation; Endotoxin; septic shock; Peptidoglycan

[Background] Currently, there is no method of accurately diagnosing bacterial translocation (BT) in humans. Bt may be related to changes in intestinal permeability, Changes in small-intestinal permeability caused by polyethylene glycol(PEG)have commonly been reorted in patients with chronic disease, but not in those with acute diseases. PEG does not exist in vivo. Permeability has been confirmed by measuring urinary excretion, In this study, we examined intestinal tract permeability under conditions required for BT development by using PEG.[Results] Intestinal permeability was enhanced by endotoxin stimulation in a dose-dependent manner. An increase in intestinal permeability was correlated with the incidence of BT, In the hemorrhagic shock model, oxygen administration inhibited BT development, and intestinal permeability also showed a similar tendency. Therefore, BT may develop extremely quickly, associated with enhancement of intestinal permeability of non-absorbable substances. Blood PEG (4000) levels correlated positively with the grade of invasion and the incidence of BT (Journal of the American College of Surgeons). We also reported the 2 clinical cases, which developed septic shock, believed to have been directly caused by bacterial translocation (Surg Today, Jpn 3 Surg)

[背景] 目前，尚无准确诊断人类细菌易位（BT）的方法。 Bt可能与肠道通透性变化有关，聚乙二醇（PEG）引起的小肠通透性变化通常在慢性疾病患者中出现，但在急性疾病患者中则不然。 PEG在体内不存在。通过测量尿液排泄来确认渗透性。在本研究中，我们使用 PEG 检查了 BT 发育所需条件下的肠道渗透性。[结果]内毒素刺激以剂量依赖性方式增强了肠道渗透性。肠道通透性增加与BT的发生相关，在失血性休克模型中，吸氧抑制BT的发生，肠道通透性也表现出类似的趋势。因此，BT可能发展得非常快，与不可吸收物质的肠道通透性增强有关。血液 PEG (4000) 水平与侵袭等级和 BT 发生率呈正相关（美国外科医生学会杂志）。我们还报告了 2 例出现感染性休克的临床病例，据信是由细菌移位直接引起的（Surg Today，Jpn 3 Surg）

项目成果

来見良誠: "感染巣の検索が困難であった外科感染症の1例" 日本外科感染症. vol.8. 237-241 (1996)

Yoshiaki Kurumi：“很难找到感染灶的外科感染案例”《日本外科传染病》第 8 卷（1996 年）。

Fujino Mitsuhiro: "Inhibitory effect of ulinastation on bacterial translocation mice" The Immune Consequences of Trauma, Shock and Sepsis Mechanisms and Therapeutic Approaches. 1. 918-922 (1996)

Fujino Mitsuhiro：“乌司他丁对细菌易位小鼠的抑制作用”创伤、休克和脓毒症机制和治疗方法的免疫后果。

Tohru Tani 他7名: "Bacterial Translocation as a Cause of Septic Shock in Humans: A Report of Two Cases" Surgery Today. 27・. 447-449 (1997)

Tohru Tani 等 7 人：“细菌移位导致人类感染性休克：两例报告”《今日外科》27・447-449（1997 年）。

Fujino Mitsuhiro 他6名: "Inhibitory effect of ulinastatin on bacterial translocation in mice" The Immune Consequences of Trauma, Shock and Sepsis Mechanisms and Therapeutic Approaches. 1・. 918-922 (1996)

Fujino Mitsuhiro 等 6 人：“乌司他丁对小鼠细菌易位的抑制作用”创伤、休克和脓毒症机制和治疗方法的免疫后果 1・。

Tohru Tani: "Bacterial Translocation as a Cause of Septic Shock in Humans : A Report of Two Cases" Surgery Today. 27. 447-449 (1997)

Tohru Tani：“细菌易位导致人类感染性休克：两例报告”今日手术。