Taxonomic status of Fasciola in Japan : Comparison of mitochondrial NDI and COI sequences and ribosomal ITS2 sequence with Fasciola hepatica and Fasciola gigantica

日本片形吸虫的分类地位：肝片形吸虫和巨大片形吸虫的线粒体 NDI 和 COI 序列以及核糖体 ITS2 序列的比较

• 批准号: 08660357
• 负责人: ITAGAKI Tadashi
• 金额: $ 1.28万
• 依托单位: Iwate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08660357/
• 关键词: Japanese Fasciola; F.hepatica; F.gigantica; Mitochondrial DNA; Ribosomal DNA; Nucleotide sequence; COI; NDI; ITS-2

The purpose of the study is to compare nucleotide sequences of mitochondrial NDI and COI and ribosomal ITS2 among Fasciola species, and to detect DNA polymorphism within triploid form of Fasciola in Japan. The analysis of restriction fragment length polymorphism shows that triploid Japanese Fasciola species is composed of at least two populations possessing respective type of mitochondrial DNA.It is probable that maternal origin of triploid Japanese species of the two populations is not F.hepatica, but rather F.gigantica. The one population of triploid Japanese species and diploid Korean species, both reproducing off spring parthenogenetically, would originate from the same ancestral F.gigantica. The ND1 and COI sequences of Japanese triploid forms of Fasciola were nearly identical to those found in F.gigantica, but were different from those of F.hepatica. Thus, the Japanese triploid forms of Fasciola are thought to be categorized to F.gigantica. The ITS-2 sequences of the Japanese triploid worms were divided into two distinct types, one of which was almost identical to that of F.hepatica and the other to F.gigantica from Indonesia.

该研究的目的是比较fasciola物种中线粒体NDI和COI和核糖体ITS2的核苷酸序列，并检测日本fasciola的DNA多态性。对限制片段长度多态性的分析表明，三倍体日本fasciola物种由至少两个具有各种类型的线粒体DNA的种群组成。这两个种群中的三倍体物种的母体起源可能不是f.hepatica，而是F.Hepatica，而是F.Hepatica，而是F.Hepatica，而是F.Hepatica，而是.Gigantant。一个三倍体的日本物种和二倍体韩国物种，都从春季遗传学上繁殖，源自同一祖先f.gigigantia。日本三倍体形式的筋膜的ND1和COI序列几乎与F.Gigantanta中的ND1和COI序列相同，但与F.Hepatica的ND1和COI序列不同。因此，日本的三倍体形式被认为是归类为f.gigantanta的。日本蠕虫的IT-2序列分为两种不同的类型，其中一种几乎与F.Hepatica的序列相同，而另一种与印度尼西亚的F.Gigigantia相同。

项目成果

Itagaki T.et al.: "Taxonomic status of the Japanese triploid forms of Fasciola:Comparison of mitochondrial ND1 and COI sequences with F.hepatica and F.gigantica" J.Parasitol.(In press).

Itagaki T.et al.：“日本片形吸虫三倍体形式的分类地位：线粒体 ND1 和 COI 序列与 F.hepatica 和 F.gigantica 的比较”J.Parasitol.（正在出版）。

ITagaki T et al.: "Taxonomic status of the Japanesetriploid forms of Fasciola:Comparision of mitochondrial NDI and COI sequences with F.hepatica and F.gigantica" J.Parasitol. (In press).

Itagaki T 等人：“日本片形吸虫三倍体形式的分类地位：线粒体 NDI 和 COI 序列与 F.hepatica 和 F.gigantica 的比较”J.Parasitol。

Itagaki T.: "Comparison of mitochondrial DNA sequences among Fasciola species." The Japanese Sciety for Systematic Parasitology. 15. 19-22 (1997)

Itagaki T.：“片形吸虫属物种线粒体 DNA 序列的比较。”

Itagaki T.& Tsutsumi K.: "Triploid form of Fasciola in Japan:Genetic relationships between Fasciola hepatica and Fasciola gigantica determined by ITS-2 sequence of nuclear rDNA" Int.J.Parasitol.In press.

板垣T.

板垣 匡: "ミトコンドリアDNAの塩基配列に基づいた肝蛭種間の比較" 寄生虫分類形態談話会会報. 15. 19-22 (1997)

Tadashi Itagaki：“基于线粒体 DNA 碱基序列的肝吸虫物种比较”寄生虫分类学和形态学讨论组公告。15. 19-22 (1997)。