Identification and control of ion transporter related gene (s) in crop plants

作物中离子转运蛋白相关基因的鉴定和控制

• 批准号: 08660010
• 负责人: HASEGAWA Hiroshi
• 金额: $ 1.54万
• 依托单位: The University of Shiga Prefecture
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08660010/
• 关键词: Rice; Nitrate uptake; Potassium uptake; Mutant; Root; Cesium resistance; Nitrate concentration; Differential display method; イオントランスポーター; カリウム吸収; mRNA; H^+-ATPase; 硝酸吸収; 簡易ディファレンシャル・ディスプレイ法; 突然変異体; 幼植物; ATPase; 葉緑素量

In this study the expression of gene(s) relating to nutrient ion uptake was investigated using rice mutants deficient in nitrate uptake and resistant to cesium.There was difference in the effects of inhibitors for membrane activity on both nitrate and potassium uptake between NUE5O-1, a low nitrate uptake mutant, and Kinmaze (wild type cultivar) was found. This result suggests that NUE5O-1 is a mutant defecting in ion transport system discriminating nitrate and potassium.Mutants resistant to cesium, a toxic analog for potassium, have been shown to be useful for the research on ion uptake mechanism. Six rice mutants resistant to cesium were isolated from gamma ray-mutagenized M2 population. Preliminary characterization showed that the mutants were divided into several groups. Genetics of cesium resistance has not been determined but gene products specific in the mutant roots were obtained by a simplified differential display method.Furthermore, N02/N03 assay kit (Dojindo, Japan) was shown to be a simple method for determining nitrate and nitrite content of leaves. Using the kit, nitrate metabolism in the leaves of M819, a nitrate reductase-defiecnt mutant, and NUE13-1, another low nitrate uptake mutant, was compared to that of their wild type cultivar.

本研究利用缺乏硝酸盐吸收和抗铯的水稻突变体研究了与营养离子吸收相关的基因表达。NUE5O-1 之间膜活性抑制剂对硝酸盐和钾吸收的影响存在差异。 ，一种低硝酸盐吸收突变体，和 Kinmaze（野生型品种）被发现。这一结果表明NUE5O-1是一种在区分硝酸盐和钾的离子传输系统中缺陷的突变体。对钾的有毒类似物铯具有抗性的突变体已被证明可用于离子吸收机制的研究。从伽马射线诱变的 M2 群体中分离出 6 个抗铯水稻突变体。初步表征表明突变体分为几组。铯抗性的遗传学尚未确定，但通过简化的差异显示方法获得了突变体根中特异的基因产物。此外，N02/N03测定试剂盒（Dojindo，日本）被证明是测定硝酸盐和亚硝酸盐含量的简单方法的叶子。使用该试剂盒，将硝酸盐还原酶缺陷突变体 M819 和另一种低硝酸盐吸收突变体 NUE13-1 叶子中的硝酸盐代谢与其野生型品种进行比较。

项目成果

Hasegawa, Hiroshi and Rui Takemura: "Evaluation of nitrate assimilatory ability of rice seedlings using NO2/NO3 assay kit. (In Japanese)" Kinki Journal of Crop Science and Breeding. 43. 22-26 (1998)

Hasekawa、Hiroshi 和 Rui Takemura：“使用 NO2/NO3 测定试剂盒评估水稻幼苗的硝酸盐同化能力。（日语）” Kinki Journal of Crop Science and Breeding。

Hasegawa, Hiroshi: "Recent topics of nitrate and ammonium uptake in higher plants. (In Japanese)" Kinki Journal of Crop Science and Breeding. 41. 61-65 (1996)

长谷川浩：“高等植物中硝酸盐和铵吸收的最新主题。（日语）”近畿作物科学与育种杂志。

長谷川 博: "窒素栄養の分子生物的アプローチ 1.硝酸およびアンモニアの吸収" 日本土壌肥料学雑誌. 67・4. 440-445 (1996)

长谷川浩：“氮营养的分子生物学方法1.硝酸和氨的吸收”日本土壤与肥料杂志67・4（1996）。

長谷川 博・竹村 〓: "NO2/NO3 アッセイキットを用いたイネ幼植物の硝酸代謝能の評価" 近畿作物・育種研究. 43. 22-26 (1998)

Hiroshi Hasekawa 和 Takemura：“使用 NO2/NO3 测定试剂盒评估水稻幼苗的硝酸盐代谢能力”Kinki Crop Breeding Research 43. 22-26 (1998)。

長谷川 博・竹村 塁: "NO_2/NO_3アッセイキットを用いたイネ幼植物の硝酸代謝能の評価" 近畿作物・育種研究. 43(印刷中). (1998)

Hiroshi Hasekawa 和 Rui Takemura：“使用 NO_2/NO_3 测定试剂盒评估水稻幼苗的硝酸盐代谢能力”Kinki Crop Breeding Research 43（出版中）。