Effect of different anesthetics and anesthetic exposure times on survival course of sepsis-analysis of mRNA in mice performed cecal ligation & puncture-

不同麻醉剂和麻醉剂暴露时间对盲肠结扎小鼠脓毒症生存过程的影响-mRNA分析

基本信息

批准号：
08457401
负责人：
IMAI Takasuke
金额：
$ 4.35万
依托单位：
Gunma University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

项目摘要

PURPOSE : To determine whether the survival curves and rates differ between a mutant strain of mice (C3H/HeJ : endotoxin-resistant) and endotoxin-susceptible strain (C3H/HeN) when severe sepsis (cecal ligation and puncture : CLP) is performed with different anesthetics (ketamine or varied exposure to halothane).METHODS : CLP was performed under a randomly chosen anesthetic method out of inhalation of halothane in oxygen (15 min, 2 hr, or 6 hr) (1.00,120, and 103 mice respectively) or a single injection of ketamine im (127 mice) in paired fashion in 8-week-old male mice of each strain. The daily survival rates were compared between the two strains until the 10th day after CLP for each anesthetic and among the four anesthetic methods for each strain using multiple comparisons.RESULTS : The C3H/HeJ had delayd death relative to the C3H/HeN with every anrsthetic method except a two hour halothane (p<0.01 at day 1 and day 2-5 in halothane 15 min and 6 hr, p<0.05 at day 4-6 in ketamine), however, the final survival rates for each method of anesthesia were the same in the two strains. Regardless of the genetic susceptibility to endotoxin, short exposure to halothane resulted to the most rapid death compared with the other anesthetic methods (p<0.05>, and a two hour halothane showed the best survival.CONCLUSIONS : The endotoxin susceptibility affected the septic course for each anesthetic method, and the anesthetic methods influenced the survival from sepsis in both strains.

目的：确定小鼠突变应变（C3H/HEJ：抗内毒素耐药性）和耐内毒素敏感菌株（C3H/HEN）的生存曲线和速率是否有所不同。不同的麻醉剂（氯烷基或暴露于硫烷）。方法：CLP以随机选择的麻醉方法进行，这是由于氧气中的卤代吸入（分别为15分钟，2小时或6小时）（分别为1.00,120，和103小鼠）或每种菌株的8周雄性小鼠以配对的方式单次注射氯胺酮IM（127小鼠）。在每种麻醉剂的CLP和CLP之后的第10天之间以及每种菌株的四种麻醉方法中比较了两种菌株之间的每日存活率。使用多重比较。分析：C3H/HEJ相对于每一个Anrsthetic的C3H/HEJ与C3H/HEN相对于C3H/HEN延迟死亡。方法除了两个小时的氟烷（第1天的p <0.01和硫烷15分钟和6小时的第2-5天，氯胺酮第4-6天P <0.05），但是，每种麻醉方法的最终生存率均为在这两个菌株中相同。与其他麻醉方法相比，对内毒素的遗传易感性如何，导致最快的死亡导致死亡最快（p <0.05>，两个小时的卤代硫烷均表现出最佳的生存。结论：内毒素的易感性影响了疗法的疗程。每种麻醉方法和麻醉方法都会影响两种菌株中败血症的生存。