Glyco-Signals in Regulatory Mechanisms For Proliferation, Differentiation, Senescence And Apoptosis of Hematopoietic Cells.

造血细胞增殖、分化、衰老和凋亡调节机制中的糖信号。

基本信息

  • 批准号:
    08457270
  • 负责人:
  • 金额:
    $ 4.99万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    1996
  • 资助国家:
    日本
  • 起止时间:
    1996 至 1997
  • 项目状态:
    已结题

项目摘要

During the period from 1996 to 1997, the following new findings were obtained, and most of them were presented in some of the international conferences, and published in the specialized journals : (1) The interaction of the extracellular matrix glycoprotein, fibronectin, with the integrin VLA-5 was shown to be intimately related with the apoptotic phenomena of hematopoietic cells. (2) A significant enhancement of the expression of VLA-5 mRNA was observed only in the monocytic, but not granulocytic, differentiation of human myelogenous leukemia HL-60 cells. (3) The expression of integrin VLA-5 on the cell surface was enhanced exclusively in the process of monocytic differentiation, and the most prominent expression was observed on the surface of peripheral mature monocytes. (4) The sensitivity of fibronectin-induced apoptosis was remarkably increased during monocytic differentiation, and the anti-VLA-5 monoclonal antibody or the RGD peptide completely abolished it. (5) Apoptosis of the … More normal monocytes, but not granulocytes, from peripheral blood was significantly increased by the extracellular matrix fibronectin, and the anti-fibronectin monoclonal antibody suppressed the apoptosis and increased the survival time of normal monocytes. (6) Expression of the extracellular matrix glycoprotein, tenascin, was restricted spatially and temporally during the formation of tumor tissues, depending upon the stromal-epithelial interactions, and the de novo synthesis of tenascins was induced by the soluble growth factor EGF,being also dependent on the stromal-epithelial interactions. Human tenascin C is shown to have the EGF-like domain, but this domain and its derivative peptide could not induce the expression of tenascin. (7) With all the current data taken together, it is concluded that the ECM glycoprotein fibronectin might play an important role as the negative regulator for the survival of monocytes in collaboration with the integrin VLA-5, which characteristically increases during the process of monocytic differentiation.In the latter part of the present research projects, the following novel findings were demonstrated : (8) Using the elaborately-devised expression cloning method, we have succeeded for the first time in isolating and molecularly characterizing a new and relevant gene (cDNA and its genome) which encodes a key glycosyltransferase, ganglioside GM3 synthase (sialyltransferase-1) , responsible for the biosynthesis of a ubiquitous but intriguing membraneous molecule of the simplest structure, ganglioside GM3. GM3 was previously demonstrated to exhibit a variety of biological functions intimately related to the cell growth control, malignant transformations, the cell-to-cell recognitions, and the differentiation-induction of human myelogenousleukemia cells. Less
During the period from 1996 to 1997, the following new findings were obtained, and most of them were presented in some of the international conferences, and published in the specialized journals: (1) The interaction of the extracellular matrix glycoprotein, fibronectin, with the integrin VLA-5 was shown to be intimately related with the apoptotic phenomena of hematopoietic cells. (2)仅在单核细胞中观察到VLA-5 mRNA的表达显着增强,但是(4)在单核细胞分化过程中,纤连蛋白诱导的凋亡的敏感性明显增加,抗VLA-5-5单克隆抗体或RGD完全剥离了它。 (5)…外周血的凋亡(但不是粒细胞)因细胞外基质纤连蛋白而显着增加,而抗纤维蛋白单克隆抗体抑制了凋亡,抑制了凋亡,并增加了正常单核细胞的生存时间。 (6)在肿瘤组织的形成期间,细胞外基质糖蛋白Tenascin的表达经常和临时受到限制,具体取决于基质上的上皮相互作用,替代因子EGF诱导了Teniscins的DE从NOVO合成,也依赖于基质 - 上皮相互作用。人类替痛蛋白C具有类似EGF的结构域,但该结构域及其衍生肽无法诱导替氏蛋白的表达。 (7)结论得出结论,ECM糖蛋白纤维蛋白可能起重要作用,是单核细胞与整合素VLA-5合作生存的负调节剂的重要作用方法,我们首次成功地隔离和分子表征了一种新的和相关的基因(cDNA及其基因组),该基因(cDNA及其基因组)编码了一个关键的糖基转移酶,神经节神经节苷脂GM3合酶(siAllyltransferase-1),负责使偶然剂量的杂物剂的生物合成,负责束缚二聚体的生物合成。先前证明GM3表现出与细胞生长控制,恶性转化,细胞对细胞识别以及人脊髓疾病细胞的分化诱导密切相关的多种生物学功能。

项目成果

期刊论文数量(27)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Nakamura,M., Saito,M., et al.: "Rapid Internalization of Exogenous Ganglioside GM3 and its Metabolism to Ceramide in Human Myelogenous Leukemia HL-60 Cells---" FEBS Lett.400. 350-354 (1997)
Nakamura,M.、Saito,M. 等人:“外源性神经节苷脂 GM3 的快速内化及其在人骨髓性白血病 HL-60 细胞中代谢为神经酰胺---”FEBS Lett.400。
  • DOI:
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  • 影响因子:
    0
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  • 通讯作者:
斎藤政樹: "医科分子生物学 改定第3版(村松正美、谷口維紹編)" 南江堂, 501 (1997)
齐藤正树:《医学分子生物学修订版第 3 版(村松雅美和谷口伊翔编辑)》 Nankodo,501(1997)
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  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Nakamura, M., Saito, M., et al.: "CMP-NeuAc : Galbeta1*4GlcNAcalpha2*6Sialyltransferase Catalyzes NeuAc Transfer to Glycolipids." J.Lipid Res.38. 1795-1806 (1997)
Nakamura, M.、Saito, M. 等人:“CMP-NeuAc:Galbeta1*4GlcNAcalpha2*6 唾液酸转移酶催化 NeuAc 转移到糖脂。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Ohta M,: "Suppression of hematopoietic activity in tenascin-C gene knocked-out mice" Molecular and cellular Biology. (in press). (1997)
Ohta M,:“生腱蛋白-C 基因敲除小鼠中造血活动的抑制”分子和细胞生物学。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
斎藤 政樹: "糖鎖生物学(蛋白質核酸酵素増刊号)" 共立出版株式会社(印刷中), 250 (1998)
齐藤正树:《糖生物学(蛋白质核酸酶特刊)》共立出版(正在出版),250(1998)
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  • 影响因子:
    0
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SAITO Masaki其他文献

Resourceability on Nuclear Fuel Cycle by Transmutation Approach
通过嬗变方法实现核燃料循环的资源化

SAITO Masaki的其他文献

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{{ truncateString('SAITO Masaki', 18)}}的其他基金

Molecular mechanisms of primary ciliary resorption and cilia-dependent cell cycle regulation.
原发性纤毛吸收和纤毛依赖性细胞周期调节的分子机制。
  • 批准号:
    23770136
  • 财政年份:
    2011
  • 资助金额:
    $ 4.99万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Functions of Complex Glycosphingolipids in the Cell Proliferation, Differentiation, and Cell Death Controlled at the Gene Level of Their Synthesizing Enzymes, and Their Medical Applications
复合鞘糖脂在其合成酶基因水平控制的细胞增殖、分化和细胞死亡中的功能及其医学应用
  • 批准号:
    14370310
  • 财政年份:
    2002
  • 资助金额:
    $ 4.99万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Study on Ultra-Long Life Ores Lolled with Transuranium Fuels
超长寿命矿石浸延超铀燃料的研究
  • 批准号:
    11694138
  • 财政年份:
    1999
  • 资助金额:
    $ 4.99万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Expression Mechanism and Its Medical Application of Ganglioside GM3 Synthase Gene Which Is Relevantly Related With Growth and Differentiation of Hematopoietic Cells
与造血细胞生长分化相关的神经节苷脂GM3合酶基因的表达机制及其医学应用
  • 批准号:
    10470206
  • 财政年份:
    1998
  • 资助金额:
    $ 4.99万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Nuclear Energy Systems with Zero Release of Radioactive Materials
放射性物质零释放的核能系统
  • 批准号:
    09044146
  • 财政年份:
    1997
  • 资助金额:
    $ 4.99万
  • 项目类别:
    Grant-in-Aid for international Scientific Research
Exploitation on Biological Activities of Sialoglycosphingolipids and Their Synthetic Family Compounds (Neoglycollipids)
唾液酸鞘糖脂及其合成家族化合物(新糖脂)的生物活性开发
  • 批准号:
    09359001
  • 财政年份:
    1997
  • 资助金额:
    $ 4.99万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Regulatory Mochanisms For Proliferation, Differentiation, And Apoptosis of Leukemic Cells In Reference To Cell Cycle Phases.
白血病细胞增殖、分化和凋亡与细胞周期阶段相关的调节机制。
  • 批准号:
    06454349
  • 财政年份:
    1994
  • 资助金额:
    $ 4.99万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Basic Study on MHD Power Generation System by Using High Density Liquid-Metal Two-Phase Natural Circulation
高密度液态金属两相自然循环磁流体发电系统基础研究
  • 批准号:
    04452328
  • 财政年份:
    1992
  • 资助金额:
    $ 4.99万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Effects of cdc2 gene and EMC tenasc in on regulation of growth and differentiation of hematopoietic cells
cdc2基因和EMC tenasc对造血细胞生长和分化的调控作用
  • 批准号:
    04454575
  • 财政年份:
    1992
  • 资助金额:
    $ 4.99万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Studies on Expression-Mechanism (s) of Malignant Phenotypes Using Growth-Factor Gene-Transfer Methods
使用生长因子基因转移方法研究恶性表型的表达机制
  • 批准号:
    02454521
  • 财政年份:
    1990
  • 资助金额:
    $ 4.99万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)

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Regulation of Flt 1 Splicing by Fibronectin and Integrin Signaling During Aging
衰老过程中纤连蛋白和整合素信号传导对 Flt 1 剪接的调节
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    10777172
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    2023
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Engineering a Kidney Organoid Model to Investigate Fibronectin-TGF-beta Signaling in Renal Fibrosis
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    2302580
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Mechanisms of anti-tumor activity of group A Streptococcus in pancreatic adenocarcinoma
A族链球菌抗胰腺癌的作用机制
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    10662559
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A novel mechanism of fibronectin/LILRB4 immune checkpoint in the regulation of the tumor microenvironment
纤连蛋白/LILRB4免疫检查点调节肿瘤微环境的新机制
  • 批准号:
    22K15519
  • 财政年份:
    2022
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2023 Fibronectin, Integrins and Related Molecules Gordon Research Conference and Gordon Research Seminar
2023年纤连蛋白、整合素及相关分子戈登研究会议暨戈登研究研讨会
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    10608783
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