Functional structure of human biological clock : Oscillation coupling and roles of melatonin in the two-oscillator model

人体生物钟的功能结构：双振荡器模型中的振荡耦合和褪黑激素的作用

基本信息

批准号：
08457018
负责人：
HONMA Ken-ichi
金额：
$ 4.74万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

项目摘要

Circadian rhythms as well as sleep were analyzed in healthy male subjects who spent 9 to 15 days without knowledge of time under temporal isolation facility. The light intensity was below 100 lux. The sleep-wake and rectal temperature rhythms were monitored continuously. Blood was sampled sequentially through an indwelling catether for 36 hours and plasma melatonin was determined. Immediately after the determination of baseline rhythms, the sleep-wake schedule was phase-advanced by 8 hours with an over night stay-up inbetween. Forced internal desynchronization was induced by fixing sleep phase either for l, 4 or 7 days, and then the subjects were released into free-run conditions.Entrainment of sleep-wake rhythm was judged from the initial phase of free-running sleep rhythm. The sleep rhythm was entrained in all subjects when the sleep phase was fixed for 7 days, and in half of subjects when fixed for 4 days. The sleep rhythm was not entrained at all when the sleep phase was fixed only for 1 days. On the other hand, the circadian rhythms in plasma melatonin and rectal temperature were not entrained even when the sleep phase was fixed for 7 days but the free-run periods were significantly affected.From these results, it becomes apparent that the sleep-wake rhythm can entrain to social time cues, but takes several days of transient until completely entrained. This entrainment does not seem to be mediated by the oscillation mechanism responsible for the ciracadian rhythms in plasma melatonin and rectal temperature. These findings support the hypothesis that the human biological clock consists of two self-sustained oscillators which are mutually coupled and whose zeitgebers are different.

在健康的男性受试者中分析了昼夜节律的节奏和睡眠，他们在9到15天的情况下，在暂时隔离设施下不了解时间。光强度低于100勒克斯。连续监测睡眠效果和直肠体温节奏。顺序通过留置链球进行了36小时的血液采样，并确定血浆褪黑激素。在确定基线节奏之后，睡眠醒来的时间表立即在8小时内进行了阶段，而过夜的时间则在介于两者之间。强迫内部对同步通过修复L，4或7天的睡眠阶段，然后将受试者释放到自由运行状态。从自由运行的睡眠节奏的初始阶段来判断睡眠效果节奏的植入。当固定睡眠阶段固定7天时，在所有受试者中都会夹住睡眠节奏，固定4天的受试者的一半。当睡眠阶段仅固定1天时，睡眠节奏根本没有被夹住。另一方面，即使固定睡眠阶段固定了7天，但自由运行的时期也显着影响，血浆褪黑激素和直肠温度的昼夜节律也不受到。可以夹带社交时间线索，但需要几天的短暂时间直到完全夹带。这种夹带似乎不是由导致血浆褪黑激素和直肠温度的Ciracadian节奏的振荡机制介导的。这些发现支持以下假设：人类生物时钟由两个自我维持的振荡器组成，这些振荡器是相互耦合且其Zeitgebers不同的。