MOLECULAR CELL BIOLOGICAL STUDIES ON INTRACELLULAR SYMBIOSIS

细胞内共生的分子细胞生物学研究

基本信息

  • 批准号:
    08404054
  • 负责人:
  • 金额:
    $ 21.7万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
  • 财政年份:
    1996
  • 资助国家:
    日本
  • 起止时间:
    1996 至 1998
  • 项目状态:
    已结题

项目摘要

We studied on Buchnera, intracellular symbiotic bacteria of aphids and intracellular yeast-like symbionts of brown planthoppers, the results of which are summarized as follows :1. Comparison of gene expression patterns of the bacteriocyte symbiotic systems from young and old aphids revealed that there are more than ten genes, of both host cell and Buchnera, that are specifically expressed only when the symbiotic system is kept functional. These genes include those for enzymes involved in the amino acid metabolism, confirming our previous results.2. We attained convincing evidence that Buchnera produce riboflavin and provision the vitamin to hosts.3. We estimated the genome size of Buchnera by digesting the genomic DNA with restriction enzymes whose restriction sites are rich in G/C, followed by pulse-field gel electrophoresis. It turned out that the genome size, 657kbp, is only one seventh of that of the genome of E.coli.4. Dot-blot analyses of two different genes and direct fluorimetry of the cellular DNA content revealed that Buchnera are very extraordinary bacteria with more than 100 genomic copies in a single cell. In addition, the genomic copy number of Buchnera is variable to a great extent due to morph and developmental stage of the host insect.5. Buchnera are exceptional as bacteria in that they contain a histone Hi -like protein, which is likely in interaction with the genomic DNA.6. We successfully cloned the gene for uricase of the yeast-like symbiont that is essential for planthoppers to mobilize the uric acid stored in their tissue. Also, we constructed a large scale expression system of the uricase gene by transforming E.coil with the gene ligated into an expression vector.
我们对Buchnera、蚜虫胞内共生菌和褐飞虱胞内酵母样共生菌进行了研究,主要结果如下: 1.比较年轻和年老蚜虫细菌细胞共生系统的基因表达模式表明,宿主细胞和 Buchnera 都有十多个基因,只有当共生系统保持功能时才会特异性表达。这些基因包括参与氨基酸代谢的酶基因,证实了我们之前的结果。2.我们获得了令人信服的证据表明Buchnera 产生核黄素并向宿主提供维生素。3.我们通过用限制性酶切位点富含 G/C 的限制性内切酶消化基因组 DNA,然后进行脉冲场凝胶电泳来估计 Buchnera 的基因组大小。事实证明,其基因组大小为 657kbp,仅为大肠杆菌 4 基因组的七分之一。对两种不同基因的斑点印迹分析和细胞 DNA 含量的直接荧光测定表明,Buchnera 是非常特殊的细菌,在单个细胞中拥有超过 100 个基因组拷贝。另外,Buchnera的基因组拷贝数因寄主昆虫的形态和发育阶段而存在很大差异。5. Buchnera 作为细菌的特殊之处在于它们含有组蛋白 Hi 样蛋白,该蛋白可能与基因组 DNA 相互作用。6。我们成功克隆了类酵母共生体的尿酸酶基因,该基因对于飞虱动员组织中储存的尿酸至关重要。此外,我们通过将尿酸酶基因连接到表达载体中转化大肠杆菌,构建了尿酸酶基因的大规模表达系统。

项目成果

期刊论文数量(32)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
S.Aoki: "Secondary monoecy of the gall aphid Thecabius populimonilis(Homoptera)" Japan Journal of Entomology. 64. 367-378 (1996)
S.Aoki:“胆蚜 Thecabius populimonilis(同翅目)的次生雌雄同株”,日本昆虫学杂志。
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    0
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石川 統: "生物科学入門" 裳華房, 200 (1997)
石川修:《生物科学导论》Shokabo,200(1997)
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    0
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H.Dohra, M.Fujishima, H.Ishikawa: "Structure and expressin of a groE-homologous operon of a macronucleus-specific symbiont Holospora obtusa of the ciliate Paramecium caudatum." J.Eukaryot.Microbiol.45. 71-79 (1998)
H.Dohra、M.Fujishima、H.Ishikawa:“纤毛草履虫尾部大核特异性共生体 Holospora obtusa 的 groE 同源操纵子的结构和表达。”
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    0
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H.Dohra: "Structure and expression of a groE-homologous....." Journal of Eukaryotic Microbiol.45. 71-79 (1998)
H.Dohra:“groE 同源的结构和表达......”真核微生物杂志.45。
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  • 影响因子:
    0
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H.Ishikawa: "Intracellular symbiosis in insect" Microbial Diversity in Time and Space. 93-100 (1996)
H.Ishikawa:“昆虫细胞内共生”时空微生物多样性。
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    0
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ISHIKAWA Hajime其他文献

The stranding records in Matsumae, Hokkaido during 1992-2002 (Short report)
1992-2002年北海道松前搁浅记录(短报)
  • DOI:
    10.5181/cetology.0.30_7
  • 发表时间:
    2020
  • 期刊:
  • 影响因子:
    0
  • 作者:
    MATSUDA Ayaka T.;MATSUISHI Takashi F.;ISHIKAWA Hajime;YAMADA Tadasu K.
  • 通讯作者:
    YAMADA Tadasu K.

ISHIKAWA Hajime的其他文献

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{{ truncateString('ISHIKAWA Hajime', 18)}}的其他基金

Peptide-cocktail vaccination for hepatocellular carcinoma
肽混合物疫苗接种治疗肝细胞癌
  • 批准号:
    23592000
  • 财政年份:
    2011
  • 资助金额:
    $ 21.7万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Basic Research on correlation with several Shrines Traditional Histories,in reference to Jien's Poems of Buddhist Enjoyment
与几处神社传统史相关的基础研究——以慈恩的佛乐诗为参考
  • 批准号:
    21520199
  • 财政年份:
    2009
  • 资助金额:
    $ 21.7万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
A Basic Study of Jien's One Hundred Poems of Buddhist Enjoyment, mainly in reference to the Formation of several Shrines Traditional Histories
知恩佛乐诗百首初探——主要参考几处祠堂传统史的形成
  • 批准号:
    17520124
  • 财政年份:
    2005
  • 资助金额:
    $ 21.7万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Post-genome project of the endocellular symbiotic bacterium, Buchnera.
细胞内共生细菌 Buchnera 的后基因组计划。
  • 批准号:
    12440238
  • 财政年份:
    2000
  • 资助金额:
    $ 21.7万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
General Consideration of Jien's Poetical Works of Buddhist Enjoyment
浅论继恩佛乐诗作品
  • 批准号:
    11610448
  • 财政年份:
    1999
  • 资助金额:
    $ 21.7万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
EVOLUTIONARY AND CELLH BIOLOGICAL STUDIES ON Wolbachia, ENDOCELLULAR SYMBIOTIC MICROORGANISMS
沃尔巴克氏体、细胞内共生微生物的进化和细胞生物学研究
  • 批准号:
    09044203
  • 财政年份:
    1997
  • 资助金额:
    $ 21.7万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
Evolution of Homopterae and Their intracellular Symblonts
同翅目及其细胞内符号的进化
  • 批准号:
    06044065
  • 财政年份:
    1994
  • 资助金额:
    $ 21.7万
  • 项目类别:
    Grant-in-Aid for international Scientific Research
BASIC MATERIAL OF <SHUGYOKU-SHU> TEXT (I)
<Shugyoku-Shu>文本的基本材料(I)
  • 批准号:
    05610359
  • 财政年份:
    1993
  • 资助金额:
    $ 21.7万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Molecular and Cell Biological studies on Intracellular Symbiosis
细胞内共生的分子和细胞生物学研究
  • 批准号:
    03454020
  • 财政年份:
    1991
  • 资助金额:
    $ 21.7万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Isolation, Identification and Remedial Result of An Antibiotic Substance produced by Aphid Enterobacteria
一种蚜虫肠杆菌产生的抗生素物质的分离、鉴定及治疗效果
  • 批准号:
    01840028
  • 财政年份:
    1989
  • 资助金额:
    $ 21.7万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research
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