Molecular mechanisms of pathogenicity of rinderpestvirus and its molecular epidemio1ogy

牛瘟病毒致病分子机制及其分子流行病学

基本信息

批准号：
08044226
负责人：
YAMANOUCHI Kazuya
金额：
$ 6.66万
依托单位：
Nippon Institute for Biological Science
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08044226/
关键词：
Rinderpest virus Recombinant vaccine Infection Immunity Cell-mediated immunity H protein NP protein 病原性

项目摘要

1. Studies on the efficacy of recombinant rinderpest vaccine(1) Duration of immunity by vaccinia virus expressing the H gene of rinderpest virusThe immunity induced by the virus had already shown to last for one year. The challenge test was conducted two years after the vaccination. As a result, most of the cattle vaccinated survived the challenge except few animals, indicating the duration of immunity for two years(2) Immune effect of vaccinia virus expressing the N gene of rinderpest virusAs the protective immunity by the virus was not shown in rabbits in the previous year, test was conducted on cattle. By the challenge with rinderpest of moderate level of virulence the cattle survived the challenge. However, the animals developed c1inical signs of rinderpest by the challenge with highly virulent rinderpest virus. These results indicate that the immunizing effect of the virus is lower than that by the vaccinia virus expressing the H gene.2. Cell-mediated immunity in rinderpest virus infectionAs the involvement of cell-mediated immunity was suggested for the vaccinia virus expressing the H gene, a method for cytotoxic test was developed by using the autologous target cells expressing the H gene. By this method appearance of cytotoxic T cells was confirmed at 1 week after the vaccination.An involvement of specific epitope in the development of cell-mediated immunity was also indicated for the vaccinia virus expressing the NP protein.

1。对重组剂量疫苗的疗效（1）牛ac病毒免疫的持续时间（表达Rinderpest病毒的H基因）该病毒诱导的免疫力已经持续一年。挑战测试是在接种疫苗后两年进行的。结果，除少数动物外，大多数牛接种疫苗在挑战中幸存下来，表明两年的免疫持续时间（2）表达Rinderpest病毒N基因的离发疫苗病毒的免疫作用。上一年在兔子中未显示该病毒的保护性免疫，对牛进行了测试，对牛进行了测试。通过挑战中等毒力的挑战，牛在挑战中幸存下来。但是，这些动物通过高毒性的rinderpest病毒而产生了rinderpest的c1inical迹象。这些结果表明，该病毒的免疫作用低于表达H基因的离甲酸病毒2。细胞介导的Rinderpest病毒感染的免疫力建议对表达H基因的牛仔病毒的细胞介导的免疫力参与，通过使用表达H基因的自体靶细胞开发了一种细胞毒性检测的方法。通过这种方法，在疫苗接种后的1周确认了细胞毒性T细胞的出现。特异性表位参与了表达NP蛋白的牛ac病毒的细胞介导的免疫的发育。