Biochemical and Molecular Biological S tudies on Isoprenoid

类异戊二烯的生化和分子生物学研究

• 批准号: 08044052
• 负责人: OGURA Kyozo
• 金额: $ 1.15万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08044052/
• 关键词: prenyltransferase; affinity labeling; isoprenoid; geranylgeranoicacid; dolichol; polyprenol; natural rubber

In order to promote and stimulate the mechanistic studies of enzymes involved in the biosynthesis of physiologically important isoprenoids, we invited five eminent American researchers working actively in this field to exchange information with them. Based on the result of discussion, three pieces of joint-research have been started as follows :1) Studies on the active center of prenyltransferase by means of photoaffinity labeling Collaborator : G.D.Prestwich (Utah University)2) Studies on the geranylgeranoate receptor Collabortor : Y.Shidoji (Gifu University)3) Studies on enzymens involved in the biosynthesis of natural rubber Collaborator : Y.Tanaka (Tokyo University of Agriculture and Technology)

为了促进和促进生理上重要的类异戊二烯生物合成所涉及的酶的机理研究，我们邀请了五位活跃在该领域的美国著名研究人员与他们进行信息交流。根据讨论结果，已开始以下三项联合研究：1）通过光亲和标记方法研究异戊烯基转移酶的活性中心合作者：G.D.Prestwich（犹他大学）2）香叶基香叶酸受体的研究合作者： Y.Shidoji（岐阜大学）3）天然橡胶生物合成中涉及的酶的研究合作者：Y.Tanaka （东京农工大学）

项目成果

A.Kurisaki et al.: "Proteolytic Release of Dehydrodolichyl Diphosphate Synthase from Pig Testis Microsome" Biosci.Biotech.Biochem.60. 1109-1114 (1996)

A.Kurisaki 等人：“猪睾丸微粒体中脱氢多利奇基二磷酸合酶的蛋白水解释放”Biosci.Biotech.Biochem.60。

T.Koyama et al.: "Isopentenyl Diphosphate Isomerase in Rubber Latex" Phytochemistry. 43. 769-772 (1996)

T.Koyama 等人：“橡胶乳胶中的异戊烯基二磷酸异构酶”植物化学。

K.Ogura et al: Mechanistic Enzymology and Molecular Genetics of Chain Elongation in Isoprenoid Biosynthesis. "Dynamic Aspects of Natural Products Chemintry" , Kodansha Scientific, Tokyo, K.Ohura and U.Sankawa ed., 1-23 (1996)

K.Ogura 等人：类异戊二烯生物合成中链延长的机械酶学和分子遗传学。

Y.Zhan et al.: "J.Bacteriol." Two Cistrons of the gerC Operon of Bacillus subtilis Encode the Two Subunits Heptaprenyl Diphosphate Synthase, ((in press))

Y.Zhan 等：“J.Bacteriol”。

T.Koyama et al.: "Identification of Significant Residues in the Substrate Binding Site of Farnesyl Diphosphate Synthase" Biochemistry. 35. 9533-9538 (1996)

T.Koyama 等人：“法尼基二磷酸合酶底物结合位点中重要残基的鉴定”生物化学。