Studies of cytology, enzyme electrophoresis and genomic in situ hybridization (GISH) on the evolution of Polystichum

细胞学、酶电泳和基因组原位杂交（GISH）对多穗藻进化的研究

• 批准号: 08640893
• 负责人: TAKAMIYA Masayuki
• 金额: $ 0.13万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08640893/
• 关键词: allozyme; chromosome; evolution; GISH; hybrid; polyploidy; Polystichum; pteridophyte

Chromosome number, meiotic behavior, genomic in site hybridizatio, enzyme electrophoretic studies were made on Japanese Polystichum. Polystichum Polyblepharum (Room. ex kunze) Pr., P.longifrons kurata, P.tagawanum Kurata, P.pseudo-makinoi Tagawa, P.ovatopaleacum (Kodama) Kurata, and P.makinoi (Tagawa) Tagawa were tetrads with 2n=164. Many of them displayd 82 bivalents in metaphase I,and reproduced sexually. Polystichum kiusiuensis Tagawa, P.eximium (Mett.Ex Kuhn) C.Chr. P.otomasui Sa.Kurata and P.fibrilloso-paleaceum (Kodama) Tagawa were diploids with 2n=82. In meiotic metaphase I,41 bivalents were formed in P.eximium and P.fibrilloso-paleaceum. All of putative hybrids P.*anceps Kurata, P.*hakonense kurata, P.*hatajukuense Kurata, P.*kiyozumianum Kurata, P.*pseudo-ovatopaleaceum Akasawa and P.*takaosanense Kurata were tetraploids with 2n=164 and their meiosis were irregular. Allozyme variations of sexual tetraploids displayd typical allopolyploidal patterns. Heterozygotic bands of sporophytes did not segregate in gametophyes, and they were fixed heterozygosity. Hybridities of putative hybrids were confirmed by their allozyme patterns. Genetic identity values between tetraploid species were high, while those values between diploids and tetraploids were low. GISH also confirmed genetic differences between diploid and tetraploid species.

染色体数量，减数分裂行为，基因组杂交杂交，酶电泳研究是对日本多养殖进行的。 polystichum polyblepharum（房间。他们中的许多人在中期I中显示了82个二价，并进行了性重复。 polystichum kiusiuensis tagawa，P.Espimim（Mett.ex Kuhn）C.Chr。 p.otomasui sa.kurata和P. fibrilloso-paleaceum（kodama）tagawa是二倍体，具有2n = 82。在减数分裂中期I中，在imim和p.fibrilloso-paleaceum中形成了41个二价。所有推定的混合动力p。性四倍体的同酶变异显示典型的同种多倍体模式。孢子菌素的杂合带并未在配子体中分离，它们是固定的杂合性。假定杂种的杂种通过其同酶模式证实。四倍体物种之间的遗传身份值很高，而二倍体和四倍体之间的这些值很低。 Gish还证实了二倍体和四倍体物种之间的遗传差异。