Evaluation of percutaneous absorption promoters by means of solid fractal analysis of skin stratum corneum

皮肤角质层固体分形分析评价经皮吸收促进剂

基本信息

批准号：
07672329
负责人：
TAKAYAMA Kozo
金额：
$ 1.15万
依托单位：
Hoshi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07672329/
关键词：
fractal analysis solid fractal dimension confocal laser microscope percutaneous absorption stratum corneum skin damage hairless rat absorption promoter

项目摘要

Solid fractal dimension of skin stratum corneum was determined in order to evaluate the pathological damages evoked by percutaneous absorption promoters such as d-limonene and Azone in the hairless rat. As a model dermatological system, alcoholic hydrogels containing each of these promoters were applied to the hairless rat abdominal skin. The application site of the skin was excised, fixed with glutaraldehyde and osmic acid, and then lyophilized. The skin surface was scanned with a confocal laser microscope and the image data of skin surface were fed into a computer. The skin surface structure was reconstructed as a contour mapping image on the display. The solid fractal dimension was then estimated based on the extended box-counting algorithm.The solid fractal dimension of untreated skin surface was estimated to be 2.17. The fractal dimension markedly decreased when the skin was treated with hydrogels containing d-limonene. On the other hand, the degree of decrease in fractal dimension was poor when Azone was applied to the skin. The decrease of fractal dimension may be attributed to the smoothing of the skin structure, which will be caused by the hydration and/or edema formation in the shallow part of the skin.The structure change of skin surface evoked by the promoters can be quantified as a nonintegral solid fractal dimension as well as the surface fractal dimension of skin cross section. This suggests the usefulness and reliability of the fractal analysis as a novel method for evaluating the skin damage.

确定皮肤层的固体分形维度，以评估经皮吸收启动子（如D-二烯酮和无毛大鼠）诱发的病理损害。作为模型的皮肤病系统，将包含这些启动子的每个启动子的酒精凝胶应用于无毛大鼠腹部皮肤。切除皮肤的施用部位，用戊二醛和渗透酸固定，然后冻干。用共聚焦激光显微镜扫描皮肤表面，并将皮肤表面的图像数据送入计算机。将皮肤表面结构重建为显示屏上的轮廓映射图像。然后根据扩展的盒子计数算法估算固体分形尺寸。未处理的皮肤表面的固体分形尺寸估计为2.17。当用含有D-二烯烯的水凝胶处理皮肤时，分形尺寸明显降低。另一方面，当将偶氮酮应用于皮肤时，分形维度的降低程度很差。分形尺寸的降低可能归因于皮肤结构的平滑，这将是由皮肤浅层的水合和/或水肿形成引起的。启动子唤起的皮肤表面的结构变化可以量化为非整合固体固体固体分形维度以及皮肤横截面的表面分类尺寸。这表明分形分析是评估皮肤损伤的新方法的有用性和可靠性。