Experimental Study of Tooth Replantation

牙齿再植的实验研究

基本信息

批准号：
07672099
负责人：
HAYASHI Hiroyuki
金额：
$ 1.66万
依托单位：
Osaka Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07672099/
关键词：
Tooth Replantation Storage Media Human Periodontal Ligament Cells 歯牙再植再植

项目摘要

The purpose of this study was to evaluate the effect of four solutions as storage media for tooth replantation on intracelluar LDH activity, growth activity, morphological changes of human periodontal ligament cells (PDL cells), and osmolality of solutions.The four test solutions were ViaSpan, physiological saline, human resting saliva and pasteurized milk. DMEM,including 10% FBS,was used as a control medium.The four solutions and control medium were allowed to react with PDL cells for 10,30,60 and 120 minutes respectively at 25ﾟC.The intracellular LDH activity was immediately measured after reaction of solutions. The growth activity was evaluated by the cell counts on days 1,2,4 and 7 of the recovery culture. The morphological changes were observed a phase-contrast microscope on days 1 and 7 of the recovery culture.Before performing each experiment, the osmolality of the four solutions and control medium were measured by an automatic osmometer.The results were as follows :1.The intrac … More ellular LDH activity : At all placement times, the activity of the group that reacted with ViaSpan and pasteurized milk were higher than the groups that reacted with physiological saline and human resting saliva. The groups that reacted with human resting saliva. had the lowest activity of all solutions.2.The growth activity : The groups that reacted with ViaSpan, pasteurized milk and physiological saline had high activity. In the groups that reacted with human resting saliva had the activity decreased with increasing reaction time.3.The morphological changes : The groups that reacted with ViaSpan and pasteurized milk showed almost no change in all reaction times. The groups that reacted with physiological saline became a rounded in appearance with increasing reaction time. The groups that reacted with human resting saliva appeared to shrivel and exfoliate with increasing reaction time.4.The osmolality of solution : The osmolality of human resting saliva was lowest of all solutions.5.There was a correlation among the osmolality of all solutions, the intracellular LDH activity and the growth activity of periodontal ligament cells Less

这项研究的目的是评估四种溶液作为储存培养基的影响，以使其过于重新种植对胞内LDH活性，生长活性，人类牙周韧带细胞的形态变化（PDL细胞）和溶液的渗透压。四种测试溶液是viaspan，物理盐，盐碱，盐盐盐和糊剂和糊剂。 DMEM（包括10％FBS）用作对照培养基。在25°C下，四种溶液和对照培养基分别与PDL细胞反应10,30,60分钟和120分钟。C。溶液反应后立即测量细胞内LDH活性。通过在第1,2,4天和第7天的细胞计数评估生长活性。观察到形态变化在恢复培养的第1天和第7天的相比显微镜观察到形态学变化。在执行每个实验之前，四个溶液和控制培养基的渗透率和控制培养基的渗透率是通过自动渗透压测量的。与viaspan反应和巴氏杀菌牛奶相比，与物理盐水和静息唾液反应的基团高。与人类休息的唾液反应的群体。所有溶液的活性最低。2。生长活动：与viaspan，巴氏杀菌牛奶和物理盐水反应的基团具有很高的活性。在与人静脉唾液反应的组中，活动的活性随着反应时间的增加而降低。3。形态学变化：与viaspan和巴氏杀菌牛奶反应的组在所有反应时间几乎没有变化。随着反应时间的增加，与物理盐反应的组看起来变成了圆形的外观。与人类静息唾液反应的群体似乎随着反应时间的增加而出汗和去角质。