Experimental Study of Tooth Replantation

牙齿再植的实验研究

基本信息

批准号：
07672099
负责人：
HAYASHI Hiroyuki
金额：
$ 1.66万
依托单位：
Osaka Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07672099/
关键词：
Tooth Replantation Storage Media Human Periodontal Ligament Cells 歯牙再植再植

项目摘要

The purpose of this study was to evaluate the effect of four solutions as storage media for tooth replantation on intracelluar LDH activity, growth activity, morphological changes of human periodontal ligament cells (PDL cells), and osmolality of solutions.The four test solutions were ViaSpan, physiological saline, human resting saliva and pasteurized milk. DMEM,including 10% FBS,was used as a control medium.The four solutions and control medium were allowed to react with PDL cells for 10,30,60 and 120 minutes respectively at 25ﾟC.The intracellular LDH activity was immediately measured after reaction of solutions. The growth activity was evaluated by the cell counts on days 1,2,4 and 7 of the recovery culture. The morphological changes were observed a phase-contrast microscope on days 1 and 7 of the recovery culture.Before performing each experiment, the osmolality of the four solutions and control medium were measured by an automatic osmometer.The results were as follows :1.The intrac … More ellular LDH activity : At all placement times, the activity of the group that reacted with ViaSpan and pasteurized milk were higher than the groups that reacted with physiological saline and human resting saliva. The groups that reacted with human resting saliva. had the lowest activity of all solutions.2.The growth activity : The groups that reacted with ViaSpan, pasteurized milk and physiological saline had high activity. In the groups that reacted with human resting saliva had the activity decreased with increasing reaction time.3.The morphological changes : The groups that reacted with ViaSpan and pasteurized milk showed almost no change in all reaction times. The groups that reacted with physiological saline became a rounded in appearance with increasing reaction time. The groups that reacted with human resting saliva appeared to shrivel and exfoliate with increasing reaction time.4.The osmolality of solution : The osmolality of human resting saliva was lowest of all solutions.5.There was a correlation among the osmolality of all solutions, the intracellular LDH activity and the growth activity of periodontal ligament cells Less

本研究的目的是评价四种溶液作为再植牙储存介质对细胞内LDH活性、生长活性、人牙周膜细胞（PDL细胞）形态变化以及溶液渗透压的影响。四种测试溶液分别为ViaSpan 、生理盐水、人静息唾液和巴氏灭菌奶（含10% FBS）作为对照培养基。使四种溶液和对照培养基与PDL反应。 25℃分别培养细胞10、30、60和120分钟。溶液反应后立即测量细胞内LDH活性。通过恢复培养第1、2、4和7天的细胞计数评价生长活性。在恢复培养的第1天和第7天用相差显微镜观察形态变化。在进行每个实验之前，通过自动测量四种溶液和对照培养基的渗透压。结果如下： 1.细胞内LDH活性：在所有放置时间，与ViaSpan和巴氏奶反应的组的活性高于与生理盐水和人静息唾液反应的组。与人静息唾液反应的组的活性最低。2.生长活性：与ViaSpan、巴氏奶和生理盐水反应的组中活性最高。 3.形态变化：与ViaSpan和巴氏奶反应的组在所有反应时间内几乎没有变化，与生理盐水反应的组外观呈圆形。随着反应时间的增加，与人静息唾液反应的组似乎随着反应时间的增加而皱缩和剥落。4.溶液的渗透压：人静息唾液的渗透压是最低的。 5.所有溶液的渗透压、细胞内LDH活性和牙周膜细胞生长活性均存在相关性小于