Alteration of Endothelins expression in the normal and pathologic retina

正常和病理视网膜中内皮素表达的改变

• 批准号: 07671926
• 负责人: TSUDA Yasuo
• 金额: $ 1.41万
• 依托单位: NAGASAKI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671926/
• 关键词: Endothelins; ET-1; ET-3; ET_A receptor; ET_B receptor; immunohistochemistry; ETA受容体; ETB受容体; Muller細胞; neurotransmitter

We investigated the role of Endothelins in the neurotransmission of the retina by the immunohistochemistry. The eyeballs of Spraque-Dawley albino rats were enucleated. The tissues were stained with anti-ET-1 and anti-ET-3 antibody for immunohistochemical examination. ET-1 and ET-3 expression was observed in the neural retina and retinal pigment epithelial cells except the inner segment of photoreceptor cells. ET-3 immunoreactivity was observed between the outer and inner nuclear layr nuclei. This labeling was related to Muller cells. We suggest that ET-3 function as a general intercellular messenger, either directly as a neurotransmitter or indirectly as a modulator of neurotransmission by Muller cells. The tissues were also stained with anti-ET_A anti-ET_B antibody for immunohistochemical examination. ET_A receptor was observed in the whole retina, ET_B receptor was observed in the ne rve fiber layr and the inner plexiform layr. This suggest that ET_B receptor play the role of neurotransmission in the ganglion cells.

我们调查了内皮素在免疫组织化学通过视网膜神经传递中的作用。 Spraque-Dawley白化大鼠的眼球被摘除。将组织用抗ET-1和抗ET-3抗体染色，以进行免疫组织化学检查。在神经视网膜和视网膜色素上皮细胞中观察到ET-1和ET-3表达，除了光感受器细胞的内部段。在外部核Layr核之间观察到ET-3免疫反应性。该标记与Muller细胞有关。我们建议将ET-3直接作为神经递质或间接作为Muller细胞神经传递的调节剂作为一般的细胞间信使起作用。还用抗ET_A抗ET_B抗体染色的组织进行免疫组织化学检查。在整个视网膜中观察到ET_A受体，在NE RVE纤维Layr和内丛状Layr中观察到ET_B受体。这表明ET_B受体在神经节细胞中起神经传递的作用。

项目成果

Tsuda Y, Ohira A, Amemiya T: "Reduction of endothelin expression after ischemia-reper-fusion on rat retina" Invest Ophthalmol Vis Sci. 36. 119 (1995)

Tsuda Y、Ohira A、Amemiya T：“大鼠视网膜缺血再灌注后内皮素表达减少”Invest Ophasemol Vis Sci。