The mechanism of sympathetic nerve factor for lumbago

交感神经因子治疗腰痛的机制

• 批准号: 07671608
• 负责人: WATANABE Eiichi
• 金额: $ 1.41万
• 依托单位: Fukushima Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671608/
• 关键词: Dura mater; dorsal root ganglion; lumbar sympathectomy; Substance P; Neuropeptide Y; Calcitonin gene related peptide; 交感神経; 神経ペプチド; Hot plate test; 化学的交感神経遮断

Study Design : The distribution and density of nerve innervation in the lumbar dura mater after lumbar sympathectomy were assessed in Wistar rats.Objective : To provide basic information on the interaction between sympathetic and sensory nerves in patients with low back pain.Summary and Background Data : Many studies have indicated that the sinu-vertebral nerve has an important role in innervating the tissues around the vertebrae. However, the origin, innervating pattern and connections between the nerves are still controversial. It is well known that pain is often accompanied with sympathetic symptoms and exaggerated by sympathetic stimuli. Occasionally, anesthetic block at the L2 or L3 sympathetic ganglion relieves low back pain or symptoms associated with low back pain. We assessed the changes of the density and distribution of nerve innervation of the lumber dura mater after lumbar sympathectomy.Methods : Normal adult rats were sympathectomized at L2-3 level. The threshold for ther … More mal noxious pain by Hot plate analgesia test and changes in neuropeptides in the lumbar dura mater and dorsal root ganglia using light microscopic immunohistochemistry were assessed and compared to control.Results : In Hot plate analgesia test, sympathectomized rats increased their Hot plate latency time compared to that of Sham-operated rats. Density of CGRP immunoreactive fibers in sympathectomy side of the lumbar dura mater decreased to 45.5% compared to the contra-lateral side. The number and size of CGRP immunoreactive cells in dorsal root ganglia showed no difference between sympathectomized and contra-lateral side.Conclusion :1) Sympathectomy increased the pain threshold and made the sympathectomized rats hypesthetic.2) A large numbers of sensory fibers innervated the lumbar dure mater via L2-3 sympathetic nerve in rats.3) Sympathectomy reduced the number of these nerve fibers in the lumbar dura mater.4) Sympathetic nerves may play an important role for low back pain involving the lumbar dura mater. Less

研究设计：在 Wistar 大鼠中评估腰交感神经切除术后腰部硬脑膜神经支配的分布和密度。目的：提供腰痛患者交感神经和感觉神经之间相互作用的基本信息。摘要和背景数据：许多研究表明窦椎神经在支配椎骨周围的组织中发挥着重要作用，然而，神经的起源、支配模式和连接仍然存在争议。疼痛常伴有交感神经症状，并因交感神经刺激而加剧。有时，L2或L3交感神经节的麻醉阻滞可缓解腰痛或与腰痛相关的症状。我们评估了神经支配的密度和分布的变化。腰交感神经切除术后的腰部硬脑膜。方法：通过热板镇痛试验，对正常成年大鼠进行L2-3水平的交感神经切除。结果：在热板镇痛试验中，与假手术密度组大鼠相比，交感神经切除大鼠的热板潜伏期增加。腰硬脑膜交感神经切除侧的 CGRP 免疫反应纤维数量较对侧下降至 45.5%。交感神经切除组与对侧背根神经节CGRP免疫反应细胞的数量和大小无差异。结论：1）交感神经切除增加了交感神经切除大鼠的痛阈，使交感神经切除大鼠感觉亢进。2）大量感觉纤维支配腰椎大鼠通过 L2-3 交感神经进行硬脊膜切除。3) 交感神经切除术减少了腰椎硬脊膜中这些神经纤维的数量。4) 交感神经神经可能在涉及腰硬膜的腰痛中发挥重要作用。

项目成果

渡辺栄一、菊地臣一、佐藤日出夫: "腰椎椎間板ヘルニア術後成績不良例の検討" 東日本臨整会誌. 6. 98-102 (1994)

Eiichi Watanabe、Shinichi Kikuchi、Hideo Sato：“腰椎间盘突出症手术后不良结果的研究”东日本临床骨科协会杂志 6. 98-102 (1994)。

関口泰史: "脊髄硬膜・後根神経節のペプチド陽性線維-化学的交感神経遮断後の分布の変化-(第3報)" 日整会誌. 69・8. S1594 (1995)

Yasushi Sekiguchi：“硬脊膜和背根神经节中的肽阳性纤维 - 化学交感神经阻滞后分布的变化 - （第三次报告）”日精协会杂志 69・8（1995）。

渡辺栄一、菊地臣一: "整形外科における神経根ブロックの現況" 日本臨床整形外科医会会誌. 19. 9-15 (1994)

Eiichi Watanabe、Shinichi Kikuchi：“骨科神经根阻滞的现状”日本临床骨科医师学会杂志 19. 9-15 (1994)。

Y.Sekiguchi: "An anatomic study of neuropeptide immunoreactivities in the lumbar dura mater after lumbar sympathectomy" Spine. 21 8. 925-930 (1996)

Y.Sekiguchi：“腰交感神经切除术后腰硬脑膜神经肽免疫反应性的解剖学研究”脊柱。

Y.Sekiguchi: "An anatomic study of neuropeptide immunoreactivities in the lumbar dura mater after lumbar sympathectomy" Spine. 21・8. 925-930 (1996)

Y.Sekiguchi：“腰交感神经切除术后腰椎硬脑膜中神经肽免疫反应的解剖学研究”Spine 925-930 (1996)。