Defense mechanism of glomerular visceral epithelial cells against stress

肾小球脏层上皮细胞对抗应激的防御机制

基本信息

批准号：
07807105
负责人：
YAOITA Eishin
金额：
$ 1.34万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07807105/
关键词：
glomerulus visceral epithelial cells intermediate filament desmin vimentin plectin カドヘリン

项目摘要

Glomerular visceral epithelial cells (VECs) in mammals contain abundant intermediate filaments (IFs) in comparison with tubular epithelial cells. An extensive investigation of various species from amphibia to mammals showed that the characteristic was common to lower vertebrate, suggesting an important role of IF in VECs. Rat VECs express desmin. In Masugi nephritis, the staining intensity of desmin was parallel with the amount of injected dose of anti-GBM antisera and proteinuria. In puromycin aminonucleoside nephrosis, increase and decrease of the amount of desmin in VECs was shown to be parallel to proteinuria by Western blotting and immunostaining. These findings indicated that desmin expression in VECs was influenced by the condition under stress such as nephrosis. Plectin is an IF-associated protein which is postulated to function as a general cytoplasmic cross-linking element of IFs. Depending on cell types, plectin was found primarily either through the cytoplasm, at the cell periphery, or in the both location. VECs uniquely showed plectin attached predominantly to the perinuclear region. The cytoplasmic staining became intense in puromycine aminonucleoside nephrosis. We hypothesize that abundant IFs and uniquely distributed plectin in VECs function to resist mechanical stress produced by ultrafiltrate from slit diaphragm and pulsing capillary walls.

与管状上皮细胞相比，哺乳动物中的肾小球内脏上皮细胞（VEC）包含丰富的中间丝（IF）。对从两栖动物到哺乳动物的各种物种的广泛研究表明，该特征是降低脊椎动物的常见，这表明IF在VEC中的重要作用。大鼠vecs表达Desmin。在梅基肾炎中，脱敏蛋白的染色强度与注射剂量的抗GBM抗血清和蛋白尿的量平行。在嘌呤霉素氨基核苷的肾脏症中，通过蛋白质印迹和免疫染色显示，VEC中脱霉素量的增加和减少与蛋白尿平行。这些发现表明，VEC中的Desmin表达受到压力下的条件（例如肾脏病）的影响。 PLECTIN是一种IF相关蛋白，假定它是IFS的一般细胞质交联元件。根据细胞类型的不同，蛋白质主要是通过细胞质，细胞周围或两个位置发现的。 vecs独特地显示蛋白质主要附着在核周区域上。细胞质染色在尿氨基氨基核苷肾中变得强烈。我们假设VECS功能中丰富的IF和独特分布的蛋白质，以抵抗Slit隔膜和脉动毛细管壁的超滤产生的机械应力。