Expression of the Id helix-loop-helix proteins in human myeloid and lymphoid cells

Id 螺旋-环-螺旋蛋白在人骨髓细胞和淋巴样细胞中的表达

基本信息

批准号：
07670899
负责人：
ISHIGURO Akira
金额：
$ 1.54万
依托单位：
Teikyo University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670899/
关键词：
Id helix-loop-helix protein transcription factor differentiation mycloid leukemia lymphocytes human T-cell leukemia virus leukemia HTLV-1 differentiation myeloid leukemia

项目摘要

Id proteins are helix-loop-helix transcriptional factors that lack the basic DNA binding domain. The Id proteins have been generally reported to function as an inhibitor of cell differentiation. We examined the expression of mRNAs encoding human Id1, Id2 and Id3 by Northern hybridization in human mycloid and lymphoid cells. Mycloid leukemia cells with monoblastic and crythroid characteristics expressed Id2 mRNA.The levels of Id2 mRNA markedly increased with induction of differentiation of the mycloid blasts toward both granulocytes and macrophages. Increascs in Id2 mRNA expression were found in more differentiated blasts in fresh AML samples. In normal mycloid cells, Id2 mRNA was expressed cultured macrophages from bone marrow, and in mature granulocytes and monocytes from peripheral blood. Only small numbers of mycloid cells expressed mRNAs for Id1 (K562 crythroid mycloid cells) and Id3 (U-937 and THP-1 monoblastic cells). In contrast, cells from B-and T-cell lines expressed Id2 and/or Id3 mRNAs. Human T-cell leukemia virus-transformed cells prominently expressed only Id2 mRNA.Upon PHA-stimulation of blood normal lymphocytes, Id2 expression decreased and Id3 mRNA levels were up-regulated. These results show that Id2 protein may contribute to mycloid differentiation, and both Id2 and Id3 proteins may be involved in a pathway that controls lymphoid activation.

Id 蛋白是缺乏基本 DNA 结合域的螺旋-环-螺旋转录因子。据报道，Id 蛋白通常具有细胞分化抑制剂的功能。我们通过 Northern 杂交检测了编码人 Id1、Id2 和 Id3 的 mRNA 在人 mycloid 和淋巴样细胞中的表达。具有单核细胞和类冰细胞特征的粒细胞白血病细胞表达Id2 mRNA。随着粒细胞和巨噬细胞向粒细胞和巨噬细胞分化的诱导，Id2 mRNA的水平显着增加。在新鲜 AML 样本中分化程度较高的母细胞中发现 Id2 mRNA 表达增加。在正常的粒细胞中，Id2 mRNA 在骨髓培养的巨噬细胞以及外周血的成熟粒细胞和单核细胞中表达。只有少量的 mycloid 细胞表达 Id1（K562 类冻 mycloid 细胞）和 Id3（U-937 和 THP-1 单核细胞）的 mRNA。相反，来自 B 细胞系和 T 细胞系的细胞表达 Id2 和/或 Id3 mRNA。人T细胞白血病病毒转化的细胞仅显着表达Id2 mRNA。在PHA刺激血液正常淋巴细胞后，Id2表达下降，Id3 mRNA水平上调。这些结果表明，Id2 蛋白可能有助于粒细胞分化，并且 Id2 和 Id3 蛋白可能参与控制淋巴激活的途径。