THE FORENSIC APPLICATION OF ABO BLOOD GROUP GENES AND BIOLOGICAL SIGNIFICANCE OF THE ANTIGENS

ABO血型基因的法医应用及抗原的生物学意义

• 批准号: 07670488
• 负责人: NISHI Katsuji
• 金额: $ 1.41万
• 依托单位: SHIGA UNIVERSITY OF MEDICAL SCIENCE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670488/
• 关键词: ABO BLOOD GROUP; TRANSFERAZE GENES; TASTE BUDS CELLS; SPERMATOGENESIS; HASSALL'S CORPUSCLES; 糖鎖転移酵素遺伝子; ABO遺伝子; 人獣鑑別

The distribution of the ABO (H) and related antigens in some mammals including human was studied to resolve the biological significance of these aqntigens. Thes antigens were recognized in the male reproductive organs such as epididymidis, prostate and seminal vesicle of human, Japanese monkey, rat, and cat. The ABO related antigens were also expressed in the taste buds cells of the tongue of human, Japanese monkey, rat, dog, rabbit and cat. Form these it is presumed that the ABO and related antigens are associated with the chemoreceptor systems in the taste buds cells and spermatogenesis in the deproductive organs of male mammals. These antigens were additionally observed in the Hassall's corpuscles in the Thymus, showing a role of these antigens in the T-cell educatianal systems of the Thymus. The DNA samples obtained from human, rat, cat, mastomis and fish were ammplified by PCR using the primers prepared for human ABO genotyping. This resulindicate that in the forensic practice, the species identification is important procedure even in ABO genotyping by PCR because forensic material, such as salivaurine and seminal fluid migit be contaminated with the fluid from indoor pets.

研究了 ABO (H) 和相关抗原在包括人类在内的一些哺乳动物中的分布，以解决这些抗原的生物学意义。这些抗原在人类、日本猴、大鼠和猫的附睾、前列腺和精囊等雄性生殖器官中被识别。 ABO相关抗原也在人、日本猴、大鼠、狗、兔和猫的舌头味蕾细胞中表达。由此推测，ABO 血型和相关抗原与雄性哺乳动物的味蕾细胞中的化学感受器系统和生殖器官中的精子发生有关。这些抗原还在胸腺的哈萨尔小体中观察到，显示了这些抗原在胸腺 T 细胞教育系统中的作用。使用为人类 ABO 基因分型准备的引物，通过 PCR 扩增从人类、大鼠、猫、乳腺和鱼类获得的 DNA 样本。这再次表明，在法医实践中，即使在通过 PCR 进行 ABO 基因分型时，物种鉴定也是重要的程序，因为法医材料（例如唾液尿素和精液）可能会被室内宠物的体液污染。

项目成果

Ushiyama Ikuko: "Expression of blood group related carbohydorate antigens in salivary glands and male reproductive organs from rats, cats and humans." Proceedings of 2nd Internet World Congress on Biological Science CD ROM.2. CD ROM (1996)

Ushiyama Ikuko：“大鼠、猫和人类的唾液腺和雄性生殖器官中血型相关的碳水化合物抗原的表达。”

Nishi Katsuji: "Recent developement of blood grouping in the forensic field" The proceedings of the 5th Indo-Pacific Congress on Legal Medicine and Forensic Science. in press (1996)

Nishi Katsuji：“法医学领域血型分析的最新发展”第五届印度-太平洋法律医学和法医学大会的会议记录。

Nishi Katsuji: "Recent developments of blood grouping in the forensic field." Proceedings of 5th Indo-Pacific Congress on Legal Medicine and Forensic Science. 5(in press). (1997)

Nishi Katsuji：“法医学领域血型鉴定的最新进展。”

Ushiyama Ikuko: "ABH-related antigens participate in the spermatogenesis of cats and rats and rats." Advances in Forensic Haemogenetics. 6. 449-451 (1996)

牛山郁子：“ABH相关抗原参与猫和大鼠的精子发生。”

Nishi Katsuji: "Recent developement of blood grouping in the forensic field" The proceedings of the 5th Indo-Pacific Congress on Legal Medicine and Forensic Science. (in press). (1996)

Nishi Katsuji：“法医学领域血型分析的最新发展”第五届印度-太平洋法律医学和法医学大会的会议记录。