Development of evaluation for human exposure to highly toxic dioxin analogues using hair as an indicator sample

以头发为指标样品开展人体接触剧毒二恶英类似物的评估

基本信息

批准号：
07670468
负责人：
MIYATA Hideaki
金额：
$ 1.41万
依托单位：
Setsunan University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670468/
关键词：
Dioxin analogues PCDD PCDF Coplanar PCB Hair Pollution Incineration facility Analysis PCDF ユプラナーPCB

项目摘要

All dioxin analogues composing of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and coplanar PCBs (Co-PCBs) are very toxic. Even at environmental pollution level, it is revealed that they give a hormonal damage in women, and pointed out that they will occur immunosuppression and genital function damage in human. Therefore, in recent years, U.S.A., Canada and Europe countries have actively took measures to decrease the environmental pollution level.In this study, a new method with a combination of isotope dilution method was established for dioxin analogues in human hair. This method, which was composed of washing and cutting of hair, alkaline decomposition, hexane extraction, multi-layr silica gel column chromatography, alumina column chromatography and analysis by gas chromatograph/high resolution mass spectrometer, enable us to analyze dioxin analogues at an extra-trace level of less than pg/g with a good reproducibility.Using this method, hair samples from 80 control persons and from 60 workers in municipal solid waste (MSW) incineration facilities were analyzed. Taking the results of analytical data and questionnaire about age, diet habit, work, smoking, drinking etc., the hair was confirmed to be a good indicator sample for atmospheric burden of dioxin analogues. The worker was toxicologically exposed to dioxin analogues a few times greater than did the control, indicating the indoor environment of the facility to be more heavily polluted in comparison to the general environment. In addition, there was a difference in pollution degree among working places of the facility.From above results, it is concluded that this hair monitoring technique is a good means for the total evaluation of human exposure to dioxin analogues via many media.

所有二恶英类似物组成多氯联苯二苯二氧蛋白（PCDDS），多氯联苯二苯并呋喃（PCDFS）和共面PCB（Co-PCB）都非常有毒。即使在环境污染水平上，也发现她们对女性造成了激素损害，并指出她们将在人类中发生免疫抑制和生殖功能损害。因此，近年来，美国，加拿大和欧洲国家已经积极采取措施降低环境污染水平。在这项研究中，建立了一种与同位素稀释方法组合的新方法，用于人类头发中的二恶英类似物。该方法由洗发和剪发，碱性分解，己烷提取，多层硅胶柱色谱，氧化铝柱色谱和通过气相色谱/高分辨率质谱仪进行分析，使我们能够在额外的二恶英中进行分析 - 良好可重复性的跟踪水平小于PG/G。使用这种方法，分析了80名对照人员的头发样本和市政固体废物（MSW）焚化设施的60名工人的头发样本。取得分析数据的结果和有关年龄，饮食习惯，工作，吸烟，饮酒等的问卷调查，确认头发是二恶英类似物大气负担的良好指标样本。该工人在毒理学上与对照相比，与对照相比，该工人的室内环境比对照组大几倍，与一般环境相比，该设施的室内环境更加严重。此外，设施的工作场所之间的污染程度差异。从上面的结果，可以得出结论，这种头发监测技术是通过许多媒体对人类对二恶英类似物的全面评估的好方法。