ADVENTITIOUS BUD FORMATION FROM INTERCALARY MERISTEM AND MASS PROPAGATION OF HERBACEOUS PLANTS.

间生芽的形成和草本植物的大量繁殖。

基本信息

批准号：
07660036
负责人：
HOSOKI Takashi
金额：
$ 1.41万
依托单位：
SHIMANE UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07660036/
关键词：
MS medium Hyponex BA IBA Node culture shoot split method herbaceous plants propagation オーキシンサイトカイニンオウゴンヤグルマ草ドイツアザミキキョウオミナエシ MS 節切片スイトピー

项目摘要

Mass propagationof of herbaceous plants was coducted using nodal tissue because the tissue has capacity to produce axillary and adventitious buds. The methology btained here was very simple and convinient. Namely, shoot apices was cultured on MS medium containing 0,0.1 and 1 ppm BA for 2-3 weeks. Then stem with nodes was traversely cut or vertically if stem did not elongate. The cut pieces were subcultured on the same media and after 2-3 weeks, they were cut in the same manner. Thoreticallly one shoot apex could be multiplied more than one million shoots by repeating shoot sectioning for one year. Culture temperature and light condition were 25C and 4000 lux., respectively. Several herbaceous ornamentals were successfully propagated with the above methods. Roots were obtained on MS medium containing 0,0.1 and 1 ppm IBA after 3-4 week culture except Centaurea for which hyponex nedium was better than MS medium. Rooted plants were taken out from test tubes and acclimatized at 20C and 4000 lux light conditions for 2-3 weeks. They were then planted in the soil in the greenhouse. Seventy to nighty plants started growth and most of plants flowere normally. Thus three concentrations (0,0.1 and 1 ppm) test of BA and IBA were very effective because they covered optimum growth conditions for most of herbaceous plants. In conclusion herbaceous plants could be effectively propagated by the node and shoot split culture by our method.

草本植物的质量繁殖是使用淋巴结组织编码的，因为该组织具有产生腋窝和不定芽的能力。这里所涉及的甲基化学非常简单和令人信服。也就是说，在含有0,0.1和1 ppm ba的MS培养基上培养芽顶2-3周。然后，如果茎不拉长，则用节点茎进行横断或垂直切割。切割的碎片在同一媒体上进行了亚培养，2-3周后，它们以相同的方式切割。通过重复一年的射击分段，可以将一个超过一百万的芽乘以一百万张。培养温度和光条件分别为25c和4000 Lux。上述方法成功地传播了几种草本装饰品。在3-4周培养后，在含有0,0.1和1 ppm IBA的MS培养基上获得了根，除了Centaurea以外，Hyponex Nedium比MS培养基更好。从试管中取出生根的植物，并在20℃和4000 Lux光线条件下适应2-3周。然后将它们种植在温室的土壤中。七十至晚上的植物开始生长，大部分植物通常是流动的。因此，BA和IBA的三个浓度（0,0.1和1 ppm）非常有效，因为它们涵盖了大多数草本植物的最佳生长条件。总之，草本植物可以通过节点有效地传播，并通过我们的方法进行分裂培养。