Molecular based analyzes of distantly related interspecific and intergeneric hybrids (including somatic hybrids) for useful gene cloning

对远缘种间和属间杂种（包括体细胞杂种）进行分子分析，用于有用的基因克隆

• 批准号: 07660004
• 负责人: IMANISHI Shigeru
• 金额: $ 1.22万
• 依托单位: YAMAGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07660004/
• 关键词: Lycopersicon esculentum; Lycopersicon peruvianum; Lycopersicon chilense; Solanum lycopersicoides; interspecific hybrid; somatic hybrid; molecular marker; adventitious shoot formation ability; 遠縁交雑; 分子マーカー; インベルターゼ遺伝子; GISH; 酸性ホスファターゼ遺伝子; トマト栽

Genetic polymorphisms between tomato cultivar Lycopersicon esculentum and the remote wild species should be employed for the molecular genetic analysis of useful traits. The present study reports : (1) Efficiency of ovule selection culture method for the hybridization between tomato and 'peruvianum-complex' along with the development of plant materials for this study : (2) Identification of acid invertase gene for sucrose accumulation in backcross generations B_2, B_3 and B_4 from the cross of tomato and L.peruvianum var.humifusum by using PCR gene diagnosis : (3) Skewed segregation of molecular markers (RAPD,invertase gene and acid phosphatase gene) in backcross generations B_1, and B_2 : (4) Two closely linked RAPD markers and invertase gene located on chromosome 3 to high adventicious shoot formation gene which harbors in L.chilense PI128644, were discovered from the analysis of B_1 and B_2 : (5) Analysis of chloroplast DNA and nuclear genome of somatic hybrid and progenies between tomato and Solanum lycopersicoides by RFLP and GISH.

番茄品种番茄红素和偏远野生物种之间的遗传多态性应用于有用性状的分子遗传分析。本研究报告：（1）胚珠选择培养方法的效率用于番茄和“秘鲁尼古丁 - 复合物”之间的杂交以及本研究的植物材料的发展：（2）鉴定酸转化酶基因在后杂菌中蔗糖积累B_2，B_3和B_4来自番茄和L. peruvianum var.humifusum的十字架，通过使用PCR基因诊断：（3）分子标记物（RAPD，转化酶基因和酸性磷酸酶基因）的偏斜分离，B_1和B_1和B_2：（ 4）在3号染色体上的两个紧密连接的RAPD标记和转化酶基因与大型增加的芽形成基因，该基因在L.Chilense PI128644中含有，从B_1和B_2的分析中发现了叶绿体DNA和核基因组的分析。 RFLP和Gish通过番茄和茄licopersicoides之间的杂种和后代。

项目成果

TAKASHINA TADASHI: "Evaluation of the cross-incompatibility of 'peruvianum-complex' lines with tomato by the ovule selection method" Breeding Science. 47. 33-37 (1997)

TAKASHINA TADASHI：“通过胚珠选择方法评估‘peruvianum-complex’品系与番茄的交叉不亲和性”《育种科学》。

IMANISHI SHIGERU: "Introgression of L.peruvianum invertase gene into L.esculentum" Breeding Science. 46. 356-359 (1996)

IMANISHI SHIGERU：“L.peruvianum 转化酶基因渗入 L.esculentum”育种科学。

TAKASHINA TADASHI: "Segregation of high adventitious shoot formation ability and wild species-specific RAPD marker in BC_2F_1 progeny from interspecific hybrid between tomato and a wild species L.chilense" Breeding Science. 47(Supple.1). (1997)

TAKASHINA TADASHI：“番茄和野生物种 L.chilense 之间的种间杂交的 BC_2F_1 后代中高不定芽形成能力和野生物种特异性 RAPD 标记的分离”育种科学。

IMANISHI SHIGERU: "Development of interspecific hybrids between Lycopersicon esculentum and L.peruvianum var.humifusum and introgression of L.peruvianum invertase gene" Breeding Science. 46. 356-359 (1996)

IMANISHI SHIGERU：“番茄和 L.peruvianum var.humifusum 之间种间杂种的开发以及 L.peruvianum 转化酶基因的渗入”《育种科学》。

IMANISHI SHIGERU: "Development of interspecific hybrids between Lycopersicon esculetum and L.peruvianum var.humifusum and introgression of L.peruvianum invertase gene into L.esculentum" Breeding Science. 46. 356-359 (1996)

IMANISHI SHIGERU：“Lycopersicon esculetum 和 L.peruvianum var.humifusum 之间种间杂种的开发以及 L.peruvianum 转化酶基因渗入 L.esculentum”《育种科学》。